Interleukin-23 Promotes Natural Killer T-Cell Production of IL-17 during Rat Liver Transplantation
Interleukin-23 Promotes Natural Killer T-Cell Production of IL-17 during Rat Liver Transplantation
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DOI：
10.1016/j.transproceed.2011.01.175
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发表时间：
2011-06-01
影响因子：
0.9
通讯作者：
Qi, H. Z.
中科院分区：
文献类型：
--
作者：
Liu, X. C.;Zhai, A.;Qi, H. Z.
介绍。细胞因子白介素-17 （IL-17）是促进同种异体移植物细胞因子和趋化因子产生的关键促炎介质。除了Th17细胞外，自然杀伤T细胞（NKT）也被证明在激活后能够快速产生IL-17。采用酶联免疫吸附法（ELISA）测定同种异体肝移植组织中IL-17和IL-23的水平。流式细胞术检测移植物CD1d、CD4+细胞中IL-17阳性细胞。IL-17和IL-23在同种异体肝移植中高表达。与对照组相比，同种异体移植组的NKT细胞比例显著增加（P < 0.01）。体外用抗IL-23抗体阻断IL-23可抑制IL-17的表达升高（P < 0.01）。在大鼠原位肝移植急性排斥反应模型中，NKT细胞参与IL-23介导的IL-17的产生。
Introduction. Cytokine interleukin-17 (IL-17) is a key proinflammatory mediator promoting allograft cytokine and chemokine production. In addition to Th17 cells, natural killer T (NKT) cells have also been shown to be capable of rapidly producing IL-17 after activation.Methods. The levels of IL-17 and IL-23 of liver allografts were determined using enzyme-linked immunosorbent assay (ELISA). IL-17 positive cells in CD1d CD4+ cells of grafts were detected using flow cytometry.Results. High expression of IL-17 and IL-23 was observed in liver allografts. The ratios of NKT cells were dramatically increased in the allograft group compared with that in the control group (P < .01). In vitro, blockage of IL-23 using anti-IL-23 antibody can inhibit increasing expression of IL-17 (P < .01).Conclusion. NKT cells contribute to production of IL-17 mediated by IL-23 on a rat acute allograft rejection model of orthotopic liver transplantation (OLT).