INACTIVATION OF LASSA, MARBURG, AND EBOLA VIRUSES BY GAMMA-IRRADIATION
INACTIVATION OF LASSA, MARBURG, AND EBOLA VIRUSES BY GAMMA-IRRADIATION
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DOI：
10.1128/jcm.16.4.704-708.1982
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发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
JOHNSON, KM
中科院分区：
文献类型：
--
作者：
ELLIOTT, LH;MCCORMICK, JB;JOHNSON, KM
由于在最大封闭实验室中经历的繁琐条件，研究了灭活高致病性病毒的方法。在用60 Co γ-射线照射后，拉沙病毒、马尔堡病毒和埃博拉病毒的感染性被灭活而不改变免疫活性。射线在4.5度。C，Lassa病毒是最难消除的病毒，其速率为5.3 × 10 - 4。10-6 log 50%组织培养感染剂量[TCID 50]/rad的60 Co辐射，与6.8 × 105 Co辐射相比，10-6埃博拉病毒的log TCID 50/rad和8.4 ×10-6马尔堡病毒的log TCID 50/rad。给出了实验灭活曲线和给出灭活给定浓度的3种病毒所需的总辐射的曲线。这种灭活方法上级UV光或β-丙内酯失活，现在常规用于制备蛋白质化学研究的材料或用于制备免疫试剂。
Because of the cumbersome conditions experienced in a maximum containment laboratory, methods for inactivating highly pathogenic viruses were investigated. The infectivity of Lassa, Marburg and Ebola viruses was inactivated without altering the immunological activity after radiation with 60Co .gamma.-rays. At 4.degree. C, Lassa virus was the most difficult to inactivate with a rate of 5.3 .times. 10-6 log 50% tissue culture infective dose[TCID50]/rad of 60Co radiation, as compared with 6.8 .times. 10-6 log TCID50/rad for Ebola virus and 8.4 .times. 10-6 log TCID50/rad for Marburg virus. Experimental inactivation curves and curves giving the total radiation needed to inactivate a given concentration of any of the 3 viruses, are presented. This method of inactivation was superior to UV light or .beta.-propiolactone inactivation and is now routinely used for preparation of material for protein-chemistry studies or for preparation of immunological reagents.