Neuropathic insult increases the responsiveness to acetic acid in mice.
Neuropathic insult increases the responsiveness to acetic acid in mice.
复制标题
神经性损伤会增加小鼠对乙酸的反应性。
DOI：
10.1097/fbp.0000000000000486
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发表时间：
2019
影响因子：
1.6
通讯作者：
Damaj,MImad
中科院分区：
文献类型：
--
作者：
Gurdap,CenkO;MarkwalterJr,PatrickS;Neddenriep,Bradley;Bagdas,Deniz;Damaj,MImad
慢性神经性疼痛每天都是数百万患者的负担。神经性疼痛患者在日常生活中也会经历急性疼痛，这增加了他们的伤害性负担。本研究利用小鼠伤害性模型，探讨急性内脏痛和慢性神经性疼痛在自发性和情感性行为中的关系。采用C57BL/6J雄性小鼠坐骨神经慢性收缩损伤（CCI）诱导神经性疼痛，并通过乙酸（AA）诱导的拉伸或条件性场所厌恶实验来评估伤害和厌恶行为。与对照组相比，低浓度（0.32%）AA腹腔注射引起的拉伸在CCI和紫杉醇处理的动物中显著增加。较高浓度（1.2%）的AA均能引起神经性病变小鼠和对照组小鼠的拉伸。在条件厌恶实验中，0.32%的AA浓度对假动物和CCI动物均无诱发厌恶的作用。然而，与假小鼠相比，1.2%浓度的aa诱导CCI小鼠的地方厌恶得分更高。紫杉醇处理小鼠与灌胃小鼠在场所条件反射方面无显著差异。总的来说，我们的研究结果表明，周围神经损伤和紫杉醇治疗可引起对aa诱导的伤害感觉和地方厌恶的超敏反应。
Chronic neuropathic pain is a burden to millions of patients every day. Patients with neuropathic pain will also experience acute pain throughout their everyday lives adding to their nociceptive burden. Using nociceptive models in mice this study aimed to investigate the relationship between acute visceral pain and chronic neuropathic pain in spontaneous and affective behaviors. Neuropathic pain was induced by chronic constriction injury (CCI) of the sciatic nerve of C57BL/6J male mice and examined in assays of acetic acid (AA)-induced stretching or conditioned place aversion to assess nociceptive and aversive behaviors. Stretching induced by a low concentration (0.32%) of AA given intraperitoneally was significantly increased in CCI and paclitaxel-treated animals compared to control animals. A higher concentration (1.2%) of AA was able to induce stretching equally in both neuropathic and control mice. In the conditioned place aversion test, an AA concentration of 0.32% did not induce place aversion in either sham or CCI animals. However, the 1.2% concentration of AA-induced higher place aversion scores in CCI mice compared to sham mice. No difference in place conditioning was observed between paclitaxel and vehicle-treated mice. Overall, our results show that peripheral nerve injury and paclitaxel treatment induces hypersensitivity to AA-induced nociception and place aversion.