Matching mechanical heterogeneity of the native spinal cord augments axon infiltration in 3D-printed scaffolds
Matching mechanical heterogeneity of the native spinal cord augments axon infiltration in 3D-printed scaffolds
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DOI：
10.1016/j.biomaterials.2023.122061
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发表时间：
2023-02-25
期刊：
影响因子：
14
通讯作者：
Galie，Peter A.
中科院分区：
文献类型：
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作者：
Tran，Kiet A.;DeOre，Brandon J.;Galie，Peter A.
支架输送到受伤的脊髓，刺激轴突连接往往匹配天然组织的各向异性使用沿着头-尾轴的指导线索，但目前的方法不模仿宿主组织力学的异质性。虽然白色和灰质具有不同的机械特性，但组织力学是否也沿沿着脊髓长度变化仍不清楚。本研究中进行的力学测试表明，本体脊髓力学确实沿沿着解剖水平存在差异，这些差异是由白色和灰质比例的变化引起的。这些结果表明，重建脊髓组织力学异质性的支架必须考虑灰色和白色物质之间的差异。数字光处理（DLP）提供了一种模仿脊髓拓扑结构的方法，但以前仅限于打印均匀的机械性能。我们描述了一种方法来修改DLP打印支架，模拟脊髓的机械异质性所造成的白色和灰质的比例的变化，这改善了轴突浸润相比，控制表现出均匀的机械性能。这些结果表明，匹配白色和灰质的机械异质性的支架提高了移植到受损脊髓内的生物材料的有效性。
Scaffolds delivered to injured spinal cords to stimulate axon connectivity often match the anisotropy of native tissue using guidance cues along the rostral-caudal axis, but current approaches do not mimic the heterogeneity of host tissue mechanics. Although white and gray matter have different mechanical properties, it remains unclear whether tissue mechanics also vary along the length of the cord. Mechanical testing performed in this study indicates that bulk spinal cord mechanics do differ along anatomical level and that these differences are caused by variations in the ratio of white and gray matter. These results suggest that scaffolds recreating the heterogeneity of spinal cord tissue mechanics must account for the disparity between gray and white matter. Digital light processing (DLP) provides a means to mimic spinal cord topology, but has previously been limited to printing homogeneous mechanical properties. We describe a means to modify DLP to print scaffolds that mimic spinal cord mechanical heterogeneity caused by variation in the ratio of white and gray matter, which improves axon infiltration compared to controls exhibiting homogeneous mechanical properties. These results demonstrate that scaffolds matching the mechanical heterogeneity of white and gray matter improve the effectiveness of biomaterials transplanted within the injured spinal cord.