Effect of isomalto-oligosaccharide and gentio-oligosaccharide on the growth and fatty acid profile of Lactobacillus plantarum
Effect of isomalto-oligosaccharide and gentio-oligosaccharide on the growth and fatty acid profile of Lactobacillus plantarum
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DOI：
10.2225/vol16-issue4-fulltext-9
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发表时间：
2013-07
影响因子：
2.7
通讯作者：
C. Soto
中科院分区：
文献类型：
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作者：
C. Soto
工作背景：乳杆菌属是一类益生菌，其中一些能够通过亚油酸（LA）的生物氢化产生共轭亚油酸（CLA）。CLA和LA都是多不饱和脂肪酸，通常用于预防和控制心血管疾病、高胆固醇和癌症等疾病。碳源是一个变量，可以影响这些细菌的生长和特性。已知称为益生元的分子通过刺激存在于肠道微生物群落中的益生菌的生长和活性而有益于人类健康。本研究的目的是评估不同寡糖如何影响植物乳杆菌（NRRL - B4496）的生长和脂肪酸谱。L.在Man-Rogosa-Sharpe（MRS）培养基中进行植物乳杆菌培养，并且该培养基中的原始碳源（葡萄糖）部分或全部被寡糖（异麦芽寡糖（IMO）或龙胆寡糖（GTO））替代。采用分光光度法和气相色谱法分别测定生物量和脂肪酸组成。结果：当MRS培养基中50%的葡萄糖被IMO取代时，在37 ℃下生长最快，为2.6g/L。在相同的培养条件下，GTO的掺入仅产生2g/L的生物量。在45 ℃时，细菌培养物的生长低于在37 ℃时观察到的生长，仅达到0.4 g/L。在葡萄糖和GTO（1：1）的混合液中，于37 ℃培养，从L.植物然而，当在45 ° C下进行培养时，没有得到CLA。当使用IMO时，L.在葡萄糖或IMO存在的情况下培养的植物;然而，产生异油酸。结论：植物乳杆菌以葡萄糖和异麦芽糖的混合物为碳源生长良好。然而，这种混合物并不能提高CLA的含量，这很可能是由于促进CLA转化为异油酸的高酶活性。此外，GTO可能不太容易被该菌株代谢。因此，酶活性可能较低，较少的CLA转化为异油酸，导致CLA的积累。
Background: Lactobacillus sp. are probiotic microorganisms, and some of them are able to produce conjugated linoleic acid (CLA) via the bio-hydrogenation of linoleic acid (LA). Both CLA and LA are polyunsaturated fatty acids commonly used in the prevention and control of cardiovascular disease, high cholesterol, and cancer, among other ailments. The carbon source is one variable that can affect the growth and characteristics of these bacteria. Molecules called prebiotics are known to benefit human health by stimulating the growth and activity of probiotic bacteria present in the intestinal microflora. The aim of this study was to evaluate how different oligosaccharides affect the growth and fatty acid profile of Lactobacillus plantarum (NRRL - B4496). L. plantarum cultivation was performed in Man-Rogosa-Sharpe (MRS) medium, and the original carbon source (glucose) in this medium was partially or totally replaced by an oligosaccharide (isomaltooligosaccharide (IMO) or gentiooligosaccharide (GTO)). Then, the biomass concentration and fatty acid profile were determined using spectrophotometry and gas chromatography, respectively. Results: When 50% of the glucose in the MRS medium was replaced with IMO, the maximum growth was 2.6 g/L at 37oC. Under the same culture conditions, the incorporation of GTO only produced 2 g/L of biomass. At 45oC, the growth of the bacterial culture was lower than that observed at 37oC, reaching only 0.4 g/L. When cultivated at 37oC in a mixture of glucose and GTO (1:1), CLA (34%, c9t11) was obtained from cells of L. plantarum . However, when the cultivation was performed at 45oC, CLA was not obtained. When IMO was used, differences in CLA content were not observed between L. plantarum cultivated with glucose or with IMO present; however, vaccenic acid was produced. Conclusions: Lactobacillus plantarum grow well when a mixture of IMO and glucose is used as the carbon source. However, this mixture does not improve the CLA content, most likely due to high enzymatic activity that promotes the conversion of CLA to vaccenic acid. Additionally, GTO is likely less readily metabolized by this strain. Thus, the enzymatic activity is likely lower and less CLA is converted to vaccenic acid, resulting in an accumulation of CLA.