Generic and reversible opto-trapping of biomolecules
Generic and reversible opto-trapping of biomolecules
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DOI：
10.1016/j.actbio.2018.08.032
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发表时间：
2018-10-01
影响因子：
9.7
通讯作者：
Hoerner, Maximilian
中科院分区：
文献类型：
--
作者：
Beyer, Hannes M.;Thomas, Oliver S.;Hoerner, Maximilian
分子陷阱可以控制许多生物因子的活性和丰度。在这里，我们报告了一个通用的光陷阱的发展，可逆地结合和释放生物分子与高时空控制照明与非侵入性和细胞兼容的红色和远红光。我们使用拟南芥光感受器光敏色素B来调节各种蛋白质从各种材料支架中的释放。来自光敏色素相互作用因子6的短的100个氨基酸的“PIF-tag”的融合使得任意分子具有光陷阱相容性。可逆的光捕获的目标分子，使新的可能性，为未来的发展，在诊断，治疗，和基础research.Statement的SignificanceThe细胞信号事件的调查或复杂的治疗和综合诊断设备的发展需要刻意控制的生物分子的丰度和活性。近年来，与常规化学刺激相比，利用细胞内的天然光感受器控制不同的细胞事件，受益于光的上级空间和时间控制特性。同时，生物开关需要对生物环境的内在兼容性越来越多地发现在生物杂化材料中的应用。我们利用植物红/远红光感受器光敏色素B，它可逆地与其光敏色素相互作用因子（PIFs）相互作用，开发一个通用的光陷阱。该平台允许使用红光和远红光来时空控制任意PIF融合的生物分子从各种材料支架的结合和释放。(C)2018 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Molecular traps can control activity and abundance of many biological factors. Here, we report the development of a generic opto-trap to reversibly bind and release biomolecules with high spatiotemporal control by illumination with non-invasive and cell-compatible red and far-red light. We use the Arapidopsis thaliana photoreceptor phytochrome B to regulate the release of diverse proteins from a variety of material scaffolds. Fusion of a short 100 amino acids "PIF-tag", derived from the phytochrome interacting factor 6, renders arbitrary molecules opto-trap-compatible. Reversible opto-trapping of target molecules enables novel possibilities for future developments in diagnostics, therapeutics, and basic research.Statement of SignificanceThe investigation of cellular signaling events or the development of complex therapeutics and integrative diagnostic devices requires the deliberate control of biomolecule abundance and activity. During recent years, the use of natural photoreceptors within cells leveraged the control of diverse cellular events, benefiting from the superior spatial and temporal control characteristics of light as compared to conventional chemical stimuli. Concurrently, biological switches entailing intrinsic compatibility toward biological environments increasingly found application in biohybrid materials. We employ the plant red/far-red photoreceptor phytochrome B, which reversibly interacts with its phytochrome interacting factors (PIFs), for developing a generic opto-trap. This platform allows the use of red and far-red light to spatio-temporally control binding and release of arbitrary PIF-fused biomolecules from various material scaffolds. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.