THE YEAST SLY GENE-PRODUCTS, SUPPRESSORS OF DEFECTS IN THE ESSENTIAL GTP-BINDING YPT1 PROTEIN, MAY ACT IN ENDOPLASMIC RETICULUM-TO-GOLGI TRANSPORT
THE YEAST SLY GENE-PRODUCTS, SUPPRESSORS OF DEFECTS IN THE ESSENTIAL GTP-BINDING YPT1 PROTEIN, MAY ACT IN ENDOPLASMIC RETICULUM-TO-GOLGI TRANSPORT
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DOI：
10.1128/mcb.11.6.2980
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发表时间：
1991-06-01
影响因子：
5.3
通讯作者：
GALLWITZ, D
中科院分区：
文献类型：
--
作者：
OSSIG, R;DASCHER, C;GALLWITZ, D
It has been shown previously that defects in the essential GTP-binding protein, Ypt1p, lead to a block in protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus in the yeast Saccharomyces cerevisiae. Here we report that four newly discovered suppressors of YPT1 deletion (SLY1-20, SLY2, SLY12, and SLY41) to a varying degree restore ER-to-Golgi transport defects in cells lacking Ypt1p. These suppressors also partially complement the sec21-1 and sec22-3 mutants which lead to a defect early in the secretory pathway. Sly1p-depleted cells, as well as a conditional lethal sly2 null mutant at nonpermissive temperatures, accumulate ER membranes and core-glycosylated invertase and carboxypeptidase Y. The sly2 null mutant under restrictive conditions (37-degrees-C) can be rescued by the multicopy suppressor SLY12 and the single-copy suppressor SLY1-20, indicating that these three SLY genes functionally interact. Sly2p is shown to be an integral membrane protein.
