A multicenter study to evaluate oxidative stress by oxidation-reduction potential, a reliable and reproducible method
A multicenter study to evaluate oxidative stress by oxidation-reduction potential, a reliable and reproducible method
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DOI：
10.1111/andr.12395
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发表时间：
2017-09-01
期刊：
影响因子：
4.5
通讯作者：
Elbardisi, H.
中科院分区：
文献类型：
--
作者：
Agarwal, A.;Arafa, M.;Elbardisi, H.
众所周知，精液氧化应激（OS）会影响男性的生育能力。精子质量测量的差异阻碍了其作为精液质量指标的临床应用。有些测试测量氧化剂或还原剂的单一标记物，导致结果缺乏标准化。氧化还原电位（ORP）是OS的一个较好的代表，因为它提供了氧化剂和还原剂活性的总体度量。MiOXSYS的ORP评估已被引入作为OS的衡量标准，在区分可育和不育精液样本方面具有很高的特异性。这是一项回顾性研究，比较了美国和卡塔尔两个男科实验室在12个月期间的精液分析和ORP测量数据。两个实验室都遵循了相同的方案。美国的数据集包含194名患者和51名可生育的捐赠者，而卡塔尔的数据集包含400名患者和50名可生育的捐赠者。在这两个数据集和合并数据集中，与有生育能力的男性相比，不育组的精子浓度、总活力和渐进活力、正常形态以及更高的ORP水平都明显较低(p
Seminal oxidative stress (OS) is well-known to affect male fertility status. The discrepancy in OS measurement has hindered its clinical use as a quality indicator for semen. Some tests measured single markers of oxidants or reductants, leading to lack of standardization of results. Oxidation-reduction potential (ORP) is a better representative for OS as it provides an overall measure of the activity of both oxidants and reductants. ORP assessment by MiOXSYS has been introduced as a measure of OS with high specificity in differentiating fertile from infertile semen samples. This is a retrospective study comparing data from semen analysis and ORP measurements between two andrology laboratories in the USA and Qatar over a period of 12months. The same protocol was followed by both laboratories. The USA dataset contained 194 patients and 51 fertile donors, while the Qatar dataset contained 400 patients and 50 fertile donors. In both datasets and in the combined dataset, the infertile group had significantly lower sperm concentration, total and progressive motility, and normal morphology as well as higher ORP levels compared to fertile men (p