β-lactamase-producing strains in the species Prevotella intermedia and Prevotella nigrescens
β-lactamase-producing strains in the species Prevotella intermedia and Prevotella nigrescens
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DOI：
10.1111/j.1399-302x.1998.tb00748.x
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发表时间：
1998-02-01
影响因子：
--
通讯作者：
Mouton, C
中科院分区：
文献类型：
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作者：
Bernal, LA;Guillot, E;Mouton, C
共收集到96株菌株，包括分类为广义中间普雷沃菌的实验室菌株和临床分离株以及狭义中间普雷沃菌和变黑普雷沃菌的模式菌株。通过Etest测定对阿莫西林和阿莫西林-克拉维酸的敏感性。使用PCR-DNA探针测定将每种菌株鉴定为狭义中间青霉或变黑青霉。通过Etest，71株（74%）对阿莫西林和阿莫西林-克拉维酸敏感，最小抑菌浓度在0.016-0.064 μ g/ml范围内。相比之下，25株（26%）的阿莫西林最小抑菌浓度在1.5-96 μ g/ml范围内，同时阿莫西林-克拉维酸最小抑菌浓度在0.016-0.38 μ g/ml的低范围内，表明通过头孢硝酮试验证实了β-内酰胺酶的产生。经PCR-DNA探针法鉴定，20%（5/25）为严格意义上的中间假单胞菌，72%（18/25）为变黑假单胞菌。我们的研究结果支持了在使用β-内酰胺类抗生素治疗失败的主要作用的变黑假单胞菌。
A total of 96 strains were collected that included laboratory strains and clinical isolates classified Prevotella intermedia sensu lato and the type strains of the species P. intermedia sensu stricto and Prevotella nigrescens. Susceptibility to amoxicillin and amoxicillin-clavulanic acid was determined by the Etest. PCR-DNA probe assays were used to speciate each strain as P. intermedia sensu stricto or P. nigrescens. By Etest, 71 strains (74%) were susceptible to both amoxicillin and amoxicillin-clavulanic acid with minimum inhibitory concentrations in the 0.016-0.064 mu g/ml range. In contrast, amoxicillin minimum inhibitory concentrations of 25 strains (26%) were in the range of 1.5-96 mu g/ml with concomitant amoxicillin-clavulanic acid minimum inhibitory concentrations in the low range 0.016-0.38 mu g/ml, indicating a production of beta-lactamase as confirmed by nitrocefin tests. Of these beta-lactamase-producing strains, 20% (5/25) were identified as P. intermedia sensu stricto by the PCR-DNA probe assay and 72% (18/25) as P. nigrescens. Our results provide support for the major role of P. nigrescens in the failure of therapy using beta-lactam antibiotics.