A bioinspired Janus polyurethane membrane for potential periodontal tissue regeneration
A bioinspired Janus polyurethane membrane for potential periodontal tissue regeneration
复制标题
一种仿生 Janus 聚氨酯膜，具有潜在的牙周组织再生作用
DOI：
10.1039/d1tb02068d
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发表时间：
2021-12-09
影响因子：
7
通讯作者：
Tan, Hong
中科院分区：
文献类型：
--
作者：
He, Yushui;Wang, Xiao;Tan, Hong
引导组织再生术（GTR）是牙周组织再生的主要治疗方法。GTR策略的关键是膜，它可以帮助牙周缺损中的骨组织重建，并阻止上皮细胞和成纤维细胞向缺损迁移。然而，现有的牙周膜由于生物活性和物理化学功能有限，不能有效地促进牙周组织再生。在这里，我们开发了一种生物启发的可降解聚氨酯膜与Janus表面形态整合生物活性多巴胺（DA）和抗菌双子季铵盐（QAS）。膜的Janus表面是通过自发的微相分离制造的，这是由于具有富集的抗菌QAS的空气接触上表面和具有富集的生物活性DA的基底接触底部之间的功能段的不同迁移。用于面向软组织的上膜的光滑表面可以减少细胞粘附以抑制成纤维细胞的迁移，而面向骨的底面具有拓扑微坑结构的粗糙表面具有良好的自主矿化和细胞粘附功能，促进骨组织重建。此外，含抗菌剂QAS的膜对常见的口腔病原菌如沙门氏菌表现出良好的抗菌效果。金黄色葡萄球菌和变种人此外，特定的多巴胺基团还赋予膜优异的抗氧化效率。体内研究表明，这种Janus聚氨酯膜可以有效地促进大鼠牙周缺损模型的牙周组织再生。结合其优异的机械性能和生物相容性，聚氨酯膜是一种有前途的牙周组织再生材料。
Guided tissue regeneration (GTR) is the main therapeutic method for periodontal tissue regeneration. The key to the GTR strategy is the membrane which can assist the reconstruction of bone tissue in the periodontal defect and prevent the migration of epithelium and fibroblasts to the defect. However, the existing periodontal membrane cannot effectively promote periodontal tissue regeneration due to the limited bioactivity and physicochemical function. Here, we developed a bioinspired degradable polyurethane membrane with Janus surface morphology by integrating bioactive dopamine (DA) and an antibacterial Gemini quaternary ammonium salt (QAS). The Janus surface of the membrane is fabricated through spontaneous microphase separation, resulting from the different migration of functional segments between the air-contact upper surface with enriched antibacterial QAS and the substrate-contact bottom with enriched bioactive DA. The smooth surface of the upper membrane used to face the soft tissues can reduce cell adhesion to suppress the migration of fibroblasts, while the rough surface with a topological micro-pit structure of the bottom side facing the bone has excellent function of autonomic mineralization and cell adhesion to promote bone tissue reconstruction. In addition, the membrane containing the antibacterial QAS shows excellent antibacterial effect on common oral pathogens, such as S. aureus and S. mutans. Moreover, the specific dopamine group also endows the membrane with excellent antioxidant efficiency. In vivo research shows that this Janus polyurethane membrane can effectively promote periodontal tissue regeneration in a rat periodontal defect model. Combined with its excellent mechanical properties and biocompatibility, the polyurethane membrane is a promising material for potential periodontal tissue regeneration.