Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code: Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., S
Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code: Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., S
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两种培养和两种未培养的新盐杆菌属物种的描述，其中一种按照细菌学代码规则命名：Salinibactergrasmerensis sp。
DOI：
10.1016/j.syapm.2023.126416
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发表时间：
2023
影响因子：
3.4
通讯作者：
Mayol, Eva
中科院分区：
文献类型：
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作者：
Viver, Tomeu;Conrad, Roth E.;Lucio, Marianna;Harir, Mourad;Urdiain, Mercedes;Gago, Juan F.;Suárez-Suárez, Ana;Bustos-Caparros, Esteban;Sanchez-Martinez, Rodrigo;Mayol, Eva
目前的组学方法允许收集大量的信息，有助于描述自然界中的微生物多样性。在这里，作为培养方法的结果，从5个不同的高盐位点（在西班牙，美国和新西兰）收集了数千株分离株，我们获得了21株代表两个新的盐杆菌属物种。对于这些物种，我们建议命名为Salinibacter pegalus sp. nov.和Salinibacter grassmerensis sp. nov.。（显示与Sal的平均核苷酸同一性（ANI）值< 95.09%和87.08%。ruber M31 T），根据国际原核生物命名法（ICNP）的规则形成。代谢组学揭示了物种特异性的歧视概况。萨尔橡胶菌株的区别在于较高百分比的多不饱和脂肪酸和特定的N-官能化脂肪酸;和Sal. altiplanensis的区别在于糖基化分子数量的增加。基于宏基因组组装基因组（MAG）的序列特征和推断的表型，我们描述了盐杆菌属的两个新成员。这些物种在不同的地点占优势，并始终与萨尔共存。鲁伯和萨尔。peppery。根据阿根廷潘帕地区三个湖泊的微生物分类结果和罗马尼亚湖泊基金会的微生物分类结果，分别描述了两个湖泊中的Salinibacter pamarissp. nov.和Salinibacter abyssi sp. nov.。ruber M31 T），它们是根据序列数据描述的原核生物命名法（Code of Nomenclature of Prokaryotes Described from Sequence Data，SeqCode）提出的。这项工作构成了ICNP和SeqCode分类如何共存的一个例子。
Current-omics methods allow the collection of a large amount of information that helps in describing the microbial diversity in nature. Here, and as a result of a culturomic approach that rendered the collection of thousands of isolates from 5 different hypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains that represent two new Salinibacter species. For these species we propose the names Salinibacter pepae sp. nov. and Salinibacter grassmerensis sp. nov.(showing average nucleotide identity (ANI) values< 95.09% and 87.08% with Sal. ruber M31T, respectively), formed according to the rules of the International Code for Nomenclature of Prokaryotes (ICNP). Metabolomics revealed species-specific discriminative profiles. Sal. ruber strains were distinguished by a higher percentage of polyunsaturated fatty acids and specific N-functionalized fatty acids; and Sal. altiplanensis was distinguished by an increased number of glycosylated molecules. Based on sequence characteristics and inferred phenotype of metagenome-assembled genomes (MAGs), we describe two new members of the genus Salinibacter. These species dominated in different sites and always coexisted with Sal. ruber and Sal. pepae. Based on the MAGs from three Argentinian lakes in the Pampa region of Argentina and the MAG of the Romanian lake Fără Fund, we describe the species Salinibacter pampae sp. nov. and Salinibacter abyssi sp. nov. respectively (showing ANI values 90.94% and 91.48% with Sal. ruber M31T, respectively), which are proposed under the Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode). This work constitutes an example on how classification under ICNP and SeqCode can coexist.