Human Papillomavirus Infection and Esophageal Squamous Cell Carcinoma: A Case-Control Study
Human Papillomavirus Infection and Esophageal Squamous Cell Carcinoma: A Case-Control Study
复制标题
人乳头瘤病毒感染与食管鳞状细胞癌：病例对照研究
DOI：
10.1158/1055-9965.epi-11-1206
复制
发表时间：
2012-05-01
影响因子：
3.8
通讯作者：
Ke, Yang
中科院分区：
文献类型：
--
作者：
Guo, Fangcen;Liu, Ying;Ke, Yang
背景：中国高发地区食管鳞状细胞癌（ESCC）的危险因素尚不清楚。方法：在中国食管鳞状细胞癌高发地区安阳（中国）纳入300例食管鳞状细胞癌患者和900名年龄和性别匹配的对照者。在病例的肿瘤组织和对照的食管活检中，通过 SPF1/GP6(+) 介导的 PCR 和测序评估人乳头瘤病毒 (HPV) DNA 的存在。使用 ELISA 评估血清中针对 HPV-16 E7 癌蛋白的抗体的存在。通过无条件逻辑回归模型计算具有 95% 置信区间 (CI) 的 OR。结果：食管中 HPV 的存在（OR，6.4；95% CI，4.4-9.2）与 ESCC 风险增加相关。此外，“致癌”类型的 HPV（OR，10.3；95% Cl，6.3-16.8）感染与 ESCC 的相关性比其他类型的 HPV（OR，2.4；95% Cl，1.4-4.2）更为密切。 HPV-16（OR，12.8；95% Cl，7.6-21.7）的存在与 ESCC 密切相关。此外，与对照相比，具有 HPV-16 E7 血清抗体的病例比例更高（OR，6.1；95% CI，3.7-10.0）。结论和影响：本研究提供了迄今为止最有力的流行病学证据，支持 HPV 在中国高发地区 ESCC 发展中的重要作用。癌症流行病学生物标志物的猎物； 21(5)； 780-5。 (C)2012 AACR。
Background: The risk factors for esophageal squamous cell carcinoma (ESCC) in the high-incidence areas of China remain unclear.Methods: A total of 300 patients with ESCC and 900 controls matched for age and sex were enrolled in Anyang (China), a high-risk area for ESCC in China. In tumor tissue of the cases and in esophageal biopsies of controls, the presence of human papillomavirus (HPV) DNA was assessed by an SPF1/GP6(+)-mediated PCR followed by sequencing. The presence of serum antibody against the HPV-16 E7 oncoprotein was assessed by use of the ELISA. ORs with 95% confidence intervals (CI) were calculated via unconditional logistic regression models.Results: The presence of HPV in the esophagus (OR, 6.4; 95% Cl, 4.4-9.2) was associated with increased risk of ESCC. Moreover, infection with "oncogenic" types of HPV (OR, 10.3; 95% Cl, 6.3-16.8) was more strongly associated with ESCC than other types of HPV (OR, 2.4; 95% Cl, 1.4-4.2). The presence of HPV-16 (OR, 12.8; 95% Cl, 7.6-21.7) was particularly strongly associated with ESCC. In addition, a higher proportion of cases than controls had serum antibodies against HPV-16 E7 (OR, 6.1; 95% CI, 3.7-10.0).Conclusion and Impact: This study provides the strongest epidemiologic evidence to date in support of the important role of HPV in the development of ESCC in high-incidence areas of China. Cancer Epidemiol Biomarkers Prey; 21(5); 780-5. (C)2012 AACR.