Transcriptional analysis of human cranial compartments with different embryonic origins.
Transcriptional analysis of human cranial compartments with different embryonic origins.
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DOI：
10.1016/j.archoralbio.2015.06.008
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发表时间：
2015-09
影响因子：
3
通讯作者：
Cunningham ML
中科院分区：
文献类型：
--
作者：
Homayounfar N;Park SS;Afsharinejad Z;Bammler TK;MacDonald JW;Farin FM;Mecham BH;Cunningham ML
以往的研究表明，颅骨组织(神经脊或中胚层)的胚胎起源可能解释了缝合发育的分子机制。这项研究的目的是评估人类颅骨组织基因表达的差异，并评估是否存在反映其胚胎起源的表达特征。利用基因芯片技术，我们研究了额骨和顶骨细胞以及四个人胎颅骨中缝内间充质和矢状缝内间充质的整体基因表达。对选定的一组基因进行QRT-PCR以验证微阵列分析。对微阵列结果进行配对比较和相关分析。在六个配对比较中，额区和顶区(颅骨的不同组织类型，无论是骨还是缝内间充质)具有最不同的基因表达谱，尽管它们由相同的组织类型(骨)组成。相关分析揭示了两种不同的基因表达谱，它们将额区和后视区与顶区和矢状区分开。差异表达基因包括TFAP2A、TFAP2B、ICAM1、SULF1、TNC和FOXF2。两组组织，额区和异位区与顶区和矢状区的转录图谱显示，在增殖、分化和细胞外基质产生方面存在差异。我们的数据表明，在人类胚胎发育的中期，额室和后脑室仍然存在神经脊起源的基因表达特征，而顶室和矢状室的基因表达更类似于中胚层。
Previous investigations suggest that the embryonic origins of the calvarial tissues (neural crest or mesoderm) may account for the molecular mechanisms underlying sutural development. The aim of this study was to evaluate the differences in the gene expression of human cranial tissues and assess the presence of an expression signature reflecting their embryonic origins. Using microarray technology, we investigated global gene expression of cells from the frontal and parietal bones and the metopic and sagittal intrasutural mesenchyme (ISM) of four human foetal calvaria. qRT-PCR of a selected group of genes was done to validate the microarray analysis. Paired comparison and correlation analyses were performed on microarray results. Of six paired comparisons, frontal and parietal compartments (distinct tissue types of calvaria, either bone or intrasutural mesenchyme) had the most different gene expression profiles despite being composed of the same tissue type (bone). Correlation analysis revealed two distinct gene expression profiles that separate frontal and metopic compartments from parietal and sagittal compartments. TFAP2A, TFAP2B, ICAM1, SULF1, TNC and FOXF2 were among differentially expressed genes. Transcriptional profiles of two groups of tissues, frontal and metopic compartments vs. parietal and sagittal compartments, suggest differences in proliferation, differentiation and extracellular matrix production. Our data suggest that in the second trimester of human foetal development, a gene expression signature of neural crest origin still exists in frontal and metopic compartments while gene expression of parietal and sagittal compartments is more similar to mesoderm.