Planar embryos have poor prognosis in terms of blastocyst formation and implantation
Planar embryos have poor prognosis in terms of blastocyst formation and implantation
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DOI：
10.1016/j.rbmo.2012.05.007
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发表时间：
2012-09-01
影响因子：
4
通讯作者：
Tews, G.
中科院分区：
文献类型：
--
作者：
Ebner, T.;Maurer, M.;Tews, G.
正常情况下，第二天的胚胎表现为四个细胞的横向排列，三个卵裂球并排放置。卵裂异常包括以一个特定的平面星座为特征的胚胎，该星座由四个卵裂球组成，推测为不完全分裂。由于对这些胚胎的发育命运知之甚少，在10个月的时间里，所有连续的患者都接受了第二天平面胚胎的筛查。共检测到/2070个卵裂球构型不佳的胚胎(3.1%)。在常规体外受精中，平面胚胎的发生率(0.7%)明显低于卵胞浆内单精子注射(2.8%；P<；0.05)和睾丸精子提取(5.4%；P<；0.01)。有趣的是，卵裂异常的胚胎在第2天(P<；0.005)和第3天(P<；0.001)都表现出更好的形态。相反，四面体胚胎的囊胚形成率(P<；0.001)和囊胚质量(P=NS)较高。移植四面体胚胎的种植率明显高于必须移植平面胚胎的种植率(P<；0.01)。可以推测，在平面胚胎中，有丝分裂纺锤体可能受到了影响，例如精子中心体的组成或功能，这反过来可能导致了观察到的卵裂异常。RBM Online(C)2012，Reductive Healthcare Ltd。爱思唯尔有限公司出版。版权所有。
Normally, day-2 embryos show a crosswise arrangement of four cells with three blastomeres lying side by side. Cleavage anomalies include embryos that are characterized by a particular planar constellation of four blastomeres with presumed incomplete cleavage. Since little is known on the developmental fate of such conceptuses, within a 10-month period all consecutive patients were screened for day-2 planar embryos. A total of 64/2070 embryos with suboptimal blastomere configuration were detected (3.1%). In conventional IVF, planar embryos were significantly less frequent (0.7%) as compared with intracytoplasmic sperm injection (2.8%; P < 0.05) and cases of testicular sperm extraction (5.4%; P < 0.01). Interestingly, embryos with a cleavage anomaly showed better morphology both on day 2 (P < 0.005) and day 3 (P < 0.001). In contrast, blastocyst formation (P < 0.001) and blastocyst quality (P = NS) was higher in tetrahedral embryos. There was a significant increase in implantation rate if tetrahedral embryos could be transferred compared with when planar embryos had to be transferred (P < 0.01). It may be postulated that, in planar embryos, the mitotic spindle might have been affected, e. g. sperm centrosome composition or function, which in turn might have led to the observed cleavage anomaly. RBM Online (c) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.