Synthesis of dolichyl phosphate derivatives with fluorescent label at the omega-end of the chain, new tools to study protein glycosylation.
Synthesis of dolichyl phosphate derivatives with fluorescent label at the omega-end of the chain, new tools to study protein glycosylation.
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链欧米茄末端带有荧光标记的磷酸多乙醇酯衍生物的合成，是研究蛋白质糖基化的新工具。
DOI：
10.1016/s0960-894x(99)00662-9
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发表时间：
2000
影响因子：
2.7
通讯作者：
M. Jedrzejas
中科院分区：
文献类型：
--
作者：
V. Shibaev;V. Veselovsky;A. Lozanova;S. Maltsev;L. Danilov;W. Forsee;J. Xing;H. Cheung;M. Jedrzejas
合成了ω-末端带有2-氨基吡啶或1-氨基萘荧光基团的磷酸多酯衍生物（Dol-P）。这些产物用作重组酵母Dol-P-甘露糖合酶的底物。荧光共振能量转移之间的色氨酸残基的酶和1-aminonaphtalene组的Dol-P类似物被证明。
Derivatives of dolichyl phosphate (Dol-P) with 2-aminopyridine or 1-aminonaphtalene fluorophore groups at the ω-end of the chain were synthesized. These products serve as substrates for recombinant yeast Dol-P-mannose synthase. Fluorescence resonance energy transfer between a Trp residue of the enzyme and the 1-aminonaphtalene group of the Dol-P analogue was demonstrated.
重组酵母多甘醇磷酸甘露糖合酶的纯化和表征。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Schutzbach,JS;Zimmerman,JW;Forsee,WT
通讯作者：
Forsee,WT