Etiology and genetic evolution of canine coronavirus circulating in five provinces of China, during 2018-2019
Etiology and genetic evolution of canine coronavirus circulating in five provinces of China, during 2018-2019
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DOI：
10.1016/j.micpath.2020.104209
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发表时间：
2020-08-01
影响因子：
3.8
通讯作者：
Chen, Ye
中科院分区：
文献类型：
--
作者：
He, Hai-Jian;Zhang, Wenyan;Chen, Ye
随着新冠肺炎在全球范围内的爆发，冠状病毒再次引起了人们的关注。犬冠状病毒在犬类中广泛存在，有时甚至会导致死亡病例。在这里，为了了解当前流行的犬冠状病毒在中国地区的流行和进化情况，我们收集了2018年至2019年期间腹泻宠物狗的粪便样本213份。213份标本中检出CCoV阳性51份(23.94%)。犬细小病毒(CPV)、犬星状病毒(CaAstV)、犬柯布病毒(CaKV)、犬Teno canis病毒(TTCaV)混合感染普遍存在。不同亚型冠状病毒混合感染广泛存在。考虑到近年来有限的序列数据，我们对7个接近完整的基因组和10个完整的穗基因进行了测序。对S蛋白基因的系统发育分析发现了一个新的CCoV-II亚型，其中CCoV-IIa是目前流行最多的亚型。此外，我们还鉴定了B906_ZJ_2019株与前一株A76株的核苷酸同源性为93.24%，它们都与CCoV-II变异株聚在一起，但与已知的亚型聚在一起不是很好。B906_ZJ_2019株的重组分析表明，B906_ZJ_2019株可能是介于CCoV-I和CCoV-II之间的重组变异体，与A76株一致。此外，目前在中国流行的冠状病毒IIa株和经典的冠状病毒IIa株之间存在着广泛的氨基酸变异，尽管功能尚不清楚。总之，根据已有的序列，我们报告了关于中国冠状病毒病原和进化的有用信息，这对于制定未来有效的疾病预防和控制策略是迫切需要的。
As the outbreaks of COVID-19 in worldwide, coronavirus has once again caught the attention of people. Canine coronavirus is widespread among dog population, and sometimes causes even fatal cases. Here, to characterize the prevalence and evolution of current circulating canine coronavirus (CCoV) strains in China, we collected 213 fecal samples from diarrheic pet dogs between 2018 and 2019. Of the 213 samples, we found 51 (23.94%) were positive for CCoV. Co-infection with canine parvovirus (CPV), canine astrovirus (CaAstV), canine kobuvirus (CaKV), Torque teno canis virus (TTCaV) were ubiquitous existed. Mixed infection of different CCoV subtypes exists extensively. Considering the limited sequences data in recent years, we sequenced 7 nearly complete genomes and 10 complete spike gene. Phylogenetic analysis of spike gene revealed a new subtype CCoV-II Variant and CCoV-IIa was the most prevalent subtype currently circulating. Moreover, we identified strain B906_ZJ_2019 shared 93.24% nucleotide identifies with previous strain A76, and both of them clustered with CCoV-II Variant, which were not well clustered with the known subtypes. Recombination analysis of B906_ZJ_2019 indicated that strain B906_ZJ_2019 may a recombinant variant between CCoV-I and CCoV-II, which is consistent with strain A76. Furthermore, amino acid variations widely existed among current CCoV-IIa strains circulating in China and the classic CCoV-IIa strains, in spite of the unknown functions.In a word, we report a useful information as to the etiology and evolution of canine coronavirus in China based on the available sequences, which is urgent for the devise of future effective disease prevention and control strategies.