Gla-containing proteins of bone.
Gla-containing proteins of bone.
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DOI：
10.3109/03008208909049995
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发表时间：
1989-01-01
影响因子：
2.9
通讯作者：
Price, P A
中科院分区：
文献类型：
--
作者：
Price, P A
骨骼中含有高水平的两种蛋白质，其中含有维生素 K 依赖性 Ca2+ 结合氨基酸、γ-羧基谷氨酸 (Gla)。骨Gla蛋白（BGP，骨钙素）是一种49残基的水溶性蛋白，基质Gla蛋白（MGP）是一种79残基的水不溶性蛋白。 BGP 仅由钙化组织合成，而 MGP 由钙化组织、软骨和所有测试的软组织合成。成骨细胞中两种蛋白质的合成均受到 1,25(OH)2D3 的刺激。用维生素 K 拮抗剂华法林治疗大鼠会导致非 γ-羧基化 BGP 的分泌，该 BGP 不能与羟基磷灰石结合。华法林治疗可将骨 BGP 水平降低至正常水平的 2%，但似乎不会影响骨结构。用华法林治疗的大鼠中观察到的唯一异常是一些软骨的矿化。软骨钙化的模式与人类胎儿华法林综合征相似，可能是由于 MGP 合成异常所致。
Bone has high levels of two proteins which contain the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla). Bone Gla protein (BGP, osteocalcin) is a 49 residue water soluble protein and matrix Gla protein (MGP) is a 79 residue water insoluble protein. BGP is synthesized only by calcified tissues while MGP is synthesized by calcified tissues, cartilage, and all soft tissues tested. The synthesis of both proteins in osteoblastic cells is stimulated by 1,25(OH)2D3. Treatment of rats with the vitamin K antagonist Warfarin causes secretion of a non-gamma-carboxylated BGP which cannot bind to hydroxyapatite. Warfarin treatment reduces bone levels of BGP to 2% of normal, but does not appear to affect the structure of bone. The only abnormality seen in rats treated with Warfarin is the mineralization of several cartilages. The pattern of cartilage calcification is similar to that seen in the fetal Warfarin syndrome in humans, and may be due to abnormal synthesis of MGP.