A SIMPLE METHOD FOR DNA PURIFICATION FROM PERIPHERAL-BLOOD
A SIMPLE METHOD FOR DNA PURIFICATION FROM PERIPHERAL-BLOOD
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DOI：
10.1016/0003-2697(88)90047-4
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发表时间：
1988-11-01
影响因子：
2.9
通讯作者：
HAUSER, SL
中科院分区：
文献类型：
--
作者：
CIULLA, TA;SKLAR, RM;HAUSER, SL
本文介绍了一种新的、简单的、廉价的从全血中快速分离DNA的方法。通过细胞质膜裂解制备细胞核，用异硫氰酸胍分散核团内的DNA，并用异丙醇沉淀。用这种方法制备的DNA限制性完全，Southern分析后非特异性杂交背景低。DNA的产量与通过更繁琐的传统程序获得的产量相似。 许多基因组DNA样品可以在2小时内从全血中制备，从而促进基因连锁或其他需要大量个体的分子研究。
A new, simple, and inexpensive method for the rapid isolation of DNA from whole blood is described. Cell nuclei are prepared by lysis of cytoplasmic membranes and DNA within the nuclear pellet is dispersed with guanidine isothiocyanate and precipitated with isopropanol. DNA prepared with this way restricts completely and results in low backgrounds of nonspecific hybridization after Southern analysis. The yields of DNA are similar to those obtained by more tedious traditional procedures. Numerous genomic DNA samples can be prepared from whole blood in 2 h, thus facilitating gene linkage or other molecular studies in which large numbers of individuals are required.