Sarcolemma-specific autoantibodies in canine inflammatory myopathy
Sarcolemma-specific autoantibodies in canine inflammatory myopathy
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DOI：
10.1016/j.vetimm.2006.03.025
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发表时间：
2006-09-15
影响因子：
1.8
通讯作者：
Engvall, Eva
中科院分区：
文献类型：
--
作者：
Hankel, Sabine;Shelton, G. Diane;Engvall, Eva
炎症性肌病（IM）在犬中相对常见，在拳击手和纽芬兰犬中发病率增加。在这里，我们表明，高比例的受影响的拳击手和纽芬兰循环自身抗体对未知的肌膜抗原，这是肌肉特异性，但不是物种特异性。我们进一步表明，自身抗原可以从肌膜中提取与非离子洗涤剂，这样的洗涤剂提取物可以用于一个敏感的ELISA检测和定量抗体。在选定的犬种中，具有自身抗体的IM的相对高的发病率表明特定形式的IM的遗传易感性。在这些品种中，这种形式的IM可以通过简单的血清测定来诊断和监测。(c)2006 Elsevier B.V.保留所有权利。
Inflammatory myopathies (IM) are relatively common in dogs with an increased incidence in the Boxer and Newfoundland breeds. Here, we show that a high proportion of affected Boxers and Newfoundlands have circulating autoantibodies against unknown sarcolemma antigens, that are muscle-specific but not species specific. We further show that the autoantigen can be extracted from muscle membranes with non-ionic detergent, and that such detergent extracts can be used in a sensitive ELISA for detection and quantitation of antibodies. The relatively high incidence of IM with autoantibodies in selected breeds of dogs indicates a genetic predisposition for a particular form of IM. In these breeds, this form of IM could be diagnosed and monitored with a simple serum assay. (c) 2006 Elsevier B.V. All rights reserved.