Vacuum UV CD spectra of homopolymer duplexes and triplexes containing A.T or A.U base pairs.
Vacuum UV CD spectra of homopolymer duplexes and triplexes containing A.T or A.U base pairs.
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含有 A.T 或 A.U 碱基对的均聚物双链体和三链体的真空 UV CD 光谱。
DOI：
10.1093/nar/19.9.2275
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发表时间：
1991
影响因子：
14.9
通讯作者：
Sutherland,JC
中科院分区：
文献类型：
--
作者：
Johnson,KH;Gray,DM;Sutherland,JC
Vacuum UV circular dlchroism (CD) spectra were measured down to 174 nm for five homopolymers, five duplexes, and four triplexes containing adenlne, uracll, and thymine. Near 190 nm, the CD bands of poly[d(A)] and poly[r(A)] were larger than the CD bands of the polypyrlmidlnes, poly[d(T)], poly[d(U)], and poly[r(U)]. Little change was observed In the 190 nm region upon formation of the duplexes (poly[d(A)-d(T)], poly[d(A)-d(U)], poly[r(A)-d(T)], poly[r(A)-d(U)], and poly[r(A) r(U)]) or upon formation of two of the triplexes (poly[d(T)-d(A)-d(T)] and poly[d(U) d(A) d(U)]). This showed that the purine strand had the same or a similar structure in these duplexes and triplexes as when free in solution. Both A-U and A-T base pairing Induced positive bands at 177 and 202 nm. For three triplexes containing poly[d(A)], the formation of a triplex from a duplex and a free pyrimidine strand induced a negative band centered between 210 and 215 nm. The Induction of a band between 210 and 215 nm indicated that these triplexes had aspects of the A conformation.
