Accelerating bioelectric functional development of neural stem cells by graphene coupling: Implications for neural interfacing with conductive materials
Accelerating bioelectric functional development of neural stem cells by graphene coupling: Implications for neural interfacing with conductive materials
复制标题
通过石墨烯耦合加速神经干细胞的生物电功能发育：对神经与导电材料接口的影响。
DOI：
10.1016/j.biomaterials.2016.08.019
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发表时间：
2016-11-01
期刊：
影响因子：
14
通讯作者：
Tang, Mingliang
中科院分区：
文献类型：
--
作者：
Guo, Rongrong;Zhang, Shasha;Tang, Mingliang
为了在神经修复和再生的组织工程中控制细胞特异性行为，更好地理解材料-细胞相互作用，特别是生物电功能，是非常重要的。据报道，石墨烯是用作支架和神经接口材料的潜在候选者。然而，在这些导电石墨烯基底上的细胞膜的生物电演化在很大程度上仍然没有研究。在这项研究中，我们使用了神经干细胞（NSC）模型，以探讨可能的变化，膜生物电特性，包括静息膜电位和动作电位和细胞行为的石墨烯膜增殖和分化条件下。我们结合了单细胞电生理记录和传统的细胞生物学技术。石墨烯没有影响基本的膜电参数（电容和输入电阻），但石墨烯基板上的细胞的静息膜电位更强烈的负增殖和分化条件下。此外，与对照相比，石墨烯基底上的NSC及其后代在发育期间表现出增加的动作电位放电。然而，石墨烯仅轻微影响成熟NSC后代的电特性。石墨烯基底上的被动和主动生物电特性的调制伴随着增强的NSC分化。此外，石墨烯组的棘密度、突触蛋白表达和突触活性均增加。导电石墨烯基板上的电场的建模表明，由负电性细胞膜产生的电场在石墨烯基板上比在对照上高得多，这可能解释了通过石墨烯耦合观察到的生物电发展的变化。我们的研究结果表明，石墨烯能够加速NSC在发育过程中的成熟，特别是在生物电演化方面。我们的研究结果提供了对导电材料在石墨烯模型中调节膜生物电特性的作用的基本理解，并为未来研究开发以可控方式操纵膜特性的方法和材料铺平了道路。(C)2016爱思唯尔有限公司版权所有
In order to govern cell-specific behaviors in tissue engineering for neural repair and regeneration, a better understanding of material-cell interactions, especially the bioelectric functions, is extremely important. Graphene has been reported to be a potential candidate for use as a scaffold and neural interfacing material. However, the bioelectric evolvement of cell membranes on these conductive graphene substrates remains largely uninvestigated. In this study, we used a neural stem cell (NSC) model to explore the possible changes in membrane bioelectric properties including resting membrane potentials and action potentials and cell behaviors on graphene films under both proliferation and differentiation conditions. We used a combination of single-cell electrophysiological recordings and traditional cell biology techniques. Graphene did not affect the basic membrane electrical parameters (capacitance and input resistance), but resting membrane potentials of cells on graphene substrates were more strongly negative under both proliferation and differentiation conditions. Also, NSCs and their progeny on graphene substrates exhibited increased firing of action potentials during development compared to controls. However, graphene only slightly affected the electric characterizations of mature NSC progeny. The modulation of passive and active bioelectric properties on the graphene substrate was accompanied by enhanced NSC differentiation. Furthermore, spine density, synapse proteins expressions and synaptic activity were all increased in graphene group. Modeling of the electric field on conductive graphene substrates suggests that the electric field produced by the electronegative cell membrane is much higher on graphene substrates than that on control, and this might explain the observed changes of bioelectric development by graphene coupling. Our results indicate that graphene is able to accelerate NSC maturation during development, especially with regard to bioelectric evolvement. Our findings provide a fundamental understanding of the role of conductive materials in tuning the membrane bioelectric properties in a graphene model and pave the way for future studies on the development of methods and materials for manipulating membrane properties in a controllable way for NSC-based therapies. (C) 2016 Elsevier Ltd. All rights reserved.