KINETICS OF UPTAKE, RETENTION, AND RADIOTOXICITY OF I-125 UDR IN MAMMALIAN-CELLS - IMPLICATIONS OF LOCALIZED ENERGY DEPOSITION BY AUGER PROCESSES
KINETICS OF UPTAKE, RETENTION, AND RADIOTOXICITY OF I-125 UDR IN MAMMALIAN-CELLS - IMPLICATIONS OF LOCALIZED ENERGY DEPOSITION BY AUGER PROCESSES
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DOI：
10.2307/3576869
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发表时间：
1987-01-01
影响因子：
3.4
通讯作者：
ADELSTEIN, SJ
中科院分区：
文献类型：
--
作者：
KASSIS, AI;SASTRY, KSR;ADELSTEIN, SJ
在体外培养的哺乳动物细胞中，研究了~(125)IUdR的摄取、滞留和放射毒性动力学。DNA中的放射性与孵育时间和细胞外125I浓度成正比。细胞增殖率与细胞内放射性浓度有关，在中等浓度下细胞增殖率明显降低。0.1µCi/ml。在37%存活率时，高Let型细胞存活曲线的特征是摄取0.035 pCI/细胞，对细胞核的累积平均致死剂量约为80rad，而该细胞系的X射线剂量为580rad。~(125)I衰变对DNA的强烈杀伤作用与低能俄歇电子对DNA的局域辐照有关。
The kinetics of uptake, retention, and radiotoxicity of 125IUdR have been studied in proliferating mammalian cells in culture. The radioactivity incorporated into the DNA is directly proportional to the duration of incubation and to the extracellular concentration of 125I. The rate of proliferation of cells is related to the intracellular radioactive concentration and is markedly reduced at medium concentrations .gtoreq. 0.1 .mu.Ci/ml. At 37% survival the high LET type cell survival curve is characterized by an uptake of 0.035 pCi/cell, and the cumulated mean lethal dose to the cell nucleus is about 80 rad compared to 580 rad of X-ray dose for this cell line. The strong cytocidal effects of the decay of 125I correlate with localized irradiation of the DNA by the low energy Auger electrons.