Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells
Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells
复制标题
DOI：
10.1021/ja0466311
复制
发表时间：
2004-12-08
影响因子：
15
通讯作者：
Weisman, RB
中科院分区：
文献类型：
--
作者：
Cherukuri, P;Bachilo, SM;Weisman, RB
利用纳米管的固有近红外荧光研究了原始单壁碳纳米管进入巨噬细胞样细胞的吸收。在含有悬浮单壁纳米管的生长培养基中孵育的巨噬细胞样品显示出特征性的纳米管荧光光谱。荧光强度随着孵育时间和外纳米管浓度的增加而平稳增加。近红外荧光显微镜在1100 nm以上的波长提供了高对比度的图像，表明定位的纳米管在许多细胞内囊泡。纳米管摄取似乎通过吞噬作用发生。巨噬细胞培养物的群体生长不受暴露于单壁纳米管浓度的钙的影响。4 μg/mL，持续96 h。
The uptake of pristine single-walled carbon nanotubes into macrophage-like cells has been studied using the nanotubes' intrinsic near-infrared fluorescence. Macrophage samples that have been incubated in growth media containing suspended single-walled nanotubes show characteristic nanotube fluorescence spectra. The fluorescence intensities increase smoothly with incubation time and external nanotube concentration. Near-infrared fluorescence microscopy at wavelengths above 1100 nm provides high contrast images indicating localization of nanotubes in numerous intracellular vesicles. Nanotube uptake appears to occur through phagocytosis. Population growth of macrophage cultures is unaffected by exposure to single-walled nanotube concentrations of ca. 4 μg/mL for up to 96 h.