Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics
Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics
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DOI：
10.1021/ac0346003
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发表时间：
2003-11-15
影响因子：
7.4
通讯作者：
Vo-Dinh, T
中科院分区：
文献类型：
--
作者：
Culha, M;Stokes, D;Vo-Dinh, T
介绍了用于癌症基因检测的表面增强拉曼散射（Sers）活性基底的发展。该检测方法使用拉曼活性染料标记的DNA基因探针、自组装单分子膜和纳米结构金属基底作为SERS活性平台。在银表面上形成的巯基己烷标记的单链DNA（SH -（CH 2）（6）-ssDNA）/6-巯基-1-己醇系统通过原子力显微镜表征。本研究开发的表面增强拉曼基因（塞尔根）探针可用于通过与互补DNA探针杂交来检测DNA靶点。该探针不需要使用放射性标记，并且具有提供灵敏度和选择性的巨大潜力。该方法的有效性及其在癌症基因诊断（BRCA 1乳腺癌基因）中的应用进行了研究。
The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH - (CH2)(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microcopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.