Collagen XVII expression correlates with the invasion, and metastasis of colorectal cancer
Collagen XVII expression correlates with the invasion, and metastasis of colorectal cancer
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DOI：
10.1016/j.humpath.2014.11.020
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发表时间：
2015-03-01
期刊：
影响因子：
3.3
通讯作者：
Tasanen, Kaisa
中科院分区：
文献类型：
--
作者：
Moilanen, Jyri. M.;Kokkonen, Nina;Tasanen, Kaisa
XVII胶原蛋白作为一种黏附分子和细胞表面受体，位于复层上皮的I型半桥粒中，具有广泛的作用。它的胞外结构域从细胞表面结构性地脱落，被认为是调节皮肤上皮细胞的黏附、迁移和信号转导。之前人们认为结肠上皮细胞不表达XVII胶原蛋白。对141例结直肠癌组织芯片标本的免疫组织化学分析表明，XVI型胶原在正常大肠粘膜和结直肠癌组织中均有表达。在结直肠癌中，XVII胶原蛋白表达增加与较高的TNM分期显著相关。与浸润性生长方式、肿瘤萌芽、淋巴转移及远处转移有关。XVII胶原蛋白表达的增加与无病生存率和癌症特异性生存率的降低有关。免疫荧光染色显示，在正常组织和肿瘤组织中，XVII胶原蛋白及其已知的结合伙伴层粘连蛋白-伽马2链部分共存。在体外，小鼠XVII胶原蛋白的过表达通过Matrigel促进Caco-2结肠癌细胞的侵袭(BD Biosciences；Bedford，MA)。综上所述，本研究首次报道了结肠上皮细胞中XVI型胶原的表达，以及XVI型胶原免疫表达增强与结直肠癌预后不良的关系。(C)2015 Elsevier Inc.保留所有权利。
Collagen XVII has a well-established role as an adhesion molecule and a cell surface receptor located in the type I hemidesmosome of stratified epithelia. Its ectodomain is constitutively shed from the cell surface and suggested to regulate the adhesion, migration, and signaling of cutaneous epithelial cells. Collagen XVII was not previously thought to be expressed by colon epithelial cells. Immunohistochemical analysis of tissue microarray samples of 141 cases of colorectal carcinoma showed that collagen XVII is expressed in normal human colonic mucosa and colorectal carcinoma. In colorectal carcinoma, increased collagen XVII expression was significantly associated with higher TNM stage. It also correlated with infiltrative growth pattern and tumor budding as well as lymph node and distant metastasis. Increased collagen XVII expression was associated with decreased disease-free and cancer-specific survival. Immunofluorescence staining of collagen XVII and its well-known binding partner laminin gamma 2 chain demonstrated a partial colocalization in normal and tumor tissue. In vitro, the overexpression of murine collagen XVII promoted the invasion of CaCo-2 colon carcinoma cells through Matrigel (BD Biosciences; Bedford, MA). To conclude, this study reports for the first time the expression of collagen XVII in colon epithelium and the association of increased collagen XVII immunoexpression with poor outcome in colorectal carcinoma. (C) 2015 Elsevier Inc. All rights reserved.