DECREASE IN MACROPHAGE ANTIGEN CATABOLISM CAUSED BY AMMONIA AND CHLOROQUINE IS ASSOCIATED WITH INHIBITION OF ANTIGEN PRESENTATION TO T-CELLS
DECREASE IN MACROPHAGE ANTIGEN CATABOLISM CAUSED BY AMMONIA AND CHLOROQUINE IS ASSOCIATED WITH INHIBITION OF ANTIGEN PRESENTATION TO T-CELLS
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DOI：
10.1073/pnas.79.1.175
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
UNANUE, ER
中科院分区：
文献类型：
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作者：
ZIEGLER, HK;UNANUE, ER
本文报道氨和氯喹两种亲溶酶体化合物对小鼠单核吞噬细胞与免疫T细胞相互作用的影响。巨噬细胞对单核细胞增生李斯特菌的摄取和摄取不受药物的影响;然而，巨噬细胞对125 I标记李斯特菌的摄取以剂量依赖性方式减少。李斯特菌的巨噬细胞介绍给T细胞，一个I区依赖的现象，也被抑制。催化剂的抑制程度大于抗原呈递的抑制程度。如果在李斯特菌摄取之前和期间处理巨噬细胞，则发生抗原呈递的抑制;如果在李斯特菌摄取后30分钟处理巨噬细胞，则发生最小的抑制，此时大量的细菌已经被分解代谢。先前的研究表明，巨噬细胞相关抗原被T细胞识别后30-60分钟的李斯特菌变得明显。因此，氨和氯喹可能会影响T细胞识别相关的免疫原的表达所需的细胞内处理步骤。
The effects of 2 lysosomotropic compounds, ammonia and chloroquine, on the interaction of mononuclear phagocytes (mice) with immune T cells were described. The uptake and ingestion of Listeria monocytogenes by macrophages were not affected by the drugs; however, the macrophage catabolism of 125I-labeled Listeria was reduced in a dose-dependent way. The macrophage presentation of Listeria to T cells, an I-region-dependent phenomenon, was also inhibited. The degree of inhibition of catabolism paralleled that of antigen presentation. The inhibition of antigen presentation took place if the macrophages were treated before and during Listeria uptake; minimal inhibition took place if the macrophages were treated 30 min after Listeria ingestion, at which time a significant amount of bacteria was already catabolized. Previous studies had shown that the macrophage-associated antigen recognized by T cells became apparent 30-60 min after uptake of Listeria. Thus, ammonia and chloroquine probably affected an intracellular handling step required for the expression of the immunogen relevant for T cell recognition.