Rapid production of human KIR2DL4 extracellular domain and verification of its interaction with HLA-G
Rapid production of human KIR2DL4 extracellular domain and verification of its interaction with HLA-G
复制标题
人KIR2DL4胞外结构域的快速制备及其与HLA-G相互作用的验证
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
M. Feng
中科院分区：
文献类型：
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作者：
Yanrong Yu;Xing;Yun Wang;M. Feng
杀伤细胞免疫球蛋白样受体（KIR）2DL 4是唯一被报道由所有人类自然杀伤（NK）细胞表达的KIR成员。它在结构和功能上都不同于其他KIR成员。它与HLA-G（一种非经典的MHC I类分子）的特异性相互作用已被认为在调节NK细胞介导的细胞毒性中起重要作用。然而，这种相互作用仍然存在疑问。此外，许多研究中使用的可溶性KIR 2DL 4胞外结构域是通过真核表达产生的，其效率低于原核表达。在这项研究中，我们描述了一种方法，快速生产大量的可溶性KIR 2DL 4胞外结构域的基础上原核表达系统。利用这种可溶性KIR 2DL 4，我们验证了KIR 2DL 4和HLA-G1之间的相互作用。
Killer cell immunoglobulin-like receptor (KIR) 2DL4 is the only KIR member reported to be expressed by all human natural killer (NK) cells. It differs from other KIR members in both structure and function. Its specific interaction with HLA-G, a non-classical MHC class I molecule, has been suggested to play an important role in regulating NK cell-mediated cytotoxicity. However, this interaction is still in doubt. In addition, the soluble KIR2DL4 extracellular domain used in many studies was produced by eukaryotic expression, which is less efficient than prokaryotic expression. In this study, we describe a method of rapid production of a large amount of soluble KIR2DL4 extracellular domain based on a prokaryotic expression system. With this soluble KIR2DL4, we verified the interaction between KIR2DL4 and HLA-G1.