Limited evolution of West Nile virus has occurred during its southwesterly spread in the United States
Limited evolution of West Nile virus has occurred during its southwesterly spread in the United States
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DOI：
10.1016/s0042-6822(03)00150-8
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发表时间：
2003-05-10
期刊：
影响因子：
3.7
通讯作者：
Barrett, ADT
中科院分区：
文献类型：
--
作者：
Beasley, DWC;Davis, CT;Barrett, ADT
对2002年6月至8月在德克萨斯州东南部分离的9株西尼罗病毒株的部分核苷酸序列分析显示，与1999年纽约株的核苷酸差异最大为0.35%。鉴定出两种序列亚型，彼此相差约0.5%，表明该地区曾多次引入病毒。对一个菌株克隆PCR产物的序列分析显示，在亚群水平上，与共识序列的差异高达0.6%。迄今为止研究的北美西尼罗河病毒株中存在的少数独特突变模式可能表明，没有强大的选择压力来驱动显性变异的出现。(C) 2003 Elsevier Science（美国）版权所有。
Analysis of partial nucleotide sequences of nine West Nile virus strains isolated in southeast Texas during June-August 2002 revealed a maximum of 0.35% nucleotide variation from a New York 1999 strain. Two sequence subtypes were identified that differed from each other by approximately 0.5%, suggesting multiple introductions of virus to this area. Analysis of sequences from cloned PCR products for one strain revealed up to 0.6% divergence from the consensus sequence at the subpopulation level. The presence of unique patterns of small numbers of mutations in North American West Nile strains studied to date may suggest the absence of a strong selective pressure to drive the emergence of dominant variants. (C) 2003 Elsevier Science (USA). All rights reserved.