A pilot protein participates in the initiation of P22 procapsid assembly.
A pilot protein participates in the initiation of P22 procapsid assembly.
复制标题
引导蛋白参与 P22 衣壳组装的启动。
DOI：
10.1016/0042-6822(91)90608-e
复制
发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
PreveligeJr,P
中科院分区：
文献类型：
--
作者：
Thomas,D;PreveligeJr,P
噬菌体P22的基因16蛋白需要作为一个先导蛋白，帮助DNA从噬菌体转移到鼠伤寒沙门氏菌宿主细胞中。在组装过程中，在DNA包装之前，10-20个63,000 da gpl6蛋白拷贝被整合到原衣壳中。该蛋白是从分离的原壳蛋白中纯化出来的，在溶液中表现为单体。在体外与纯化的外壳和支架亚单位孵育后，它按照正确的化学计量量组装成原囊体。添加生理量的gpl6导致原衣壳组装率增加。涂层和gp16蛋白亚基混合物的沉降显示出关联/解离行为。添加的gp16很可能是为了稳定一种瞬时寡聚外壳蛋白，这种外壳蛋白作为原衣壳组装的体外起始复合物。
The gene 16 protein of the bacteriophage P22 is required as a pilot protein aiding the transfer of DNA from the phage into the Salmonella typhimurium host cell. During assembly 10–20 copies of the 63,000-Da gpl6 protein are incorporated into the procapsid shell prior to DNA packaging. The protein has been purified from isolated procapsids and behaved as a monomer in solution. Upon incubation with purified coat and scaffolding subunitsin vitro, it assembled into procapsids with the correct stoichiometry. The addition of physiological quantities of gpl6 resulted in an increased rate of procapsid assembly. Sedimentation of mixtures of coat and gp16 protein subunits revealed association/dissociation behavior. It is likely that the added gp16 is acting to stabilize a transient oligomeric coat protein species that functions as thein vitroinitiation complex for procapsid assembly.