Generation of induced pluripotent stem cells from human blood
Generation of induced pluripotent stem cells from human blood
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DOI：
10.1182/blood-2009-02-204800
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发表时间：
2009-05-28
期刊：
影响因子：
20.3
通讯作者：
Daley, George Q.
中科院分区：
文献类型：
--
作者：
Loh, Yuin-Han;Agarwal, Suneet;Daley, George Q.
通过皮肤活检获得的人真皮成纤维细胞可以通过特定转录因子的异位表达直接重编程为多能性。在这里，我们描述了使用OCT 4/SOX 2/KLF 4/MYC的逆转录病毒转导从CD 34（+）动员的人外周血细胞衍生诱导多能干细胞。血液来源的人诱导多能干细胞与人胚胎干细胞在形态学、表面抗原表达、多能性相关转录因子、多能细胞特异性基因的DNA甲基化状态以及体外和畸胎瘤中分化的能力方面无法区分。对来自人血液的细胞进行重编程的能力将允许产生用于疾病的患者特异性干细胞，其中致病性体细胞突变限于造血谱系的细胞。（血。2009; 113：5476-5479）
Human dermal fibroblasts obtained by skin biopsy can be reprogrammed directly to pluripotency by the ectopic expression of defined transcription factors. Here, we describe the derivation of induced pluripotent stem cells from CD34(+) mobilized human peripheral blood cells using retroviral transduction of OCT4/SOX2/KLF4/MYC. Blood-derived human induced pluripotent stem cells are indistinguishable from human embryonic stem cells with respect to morphology, expression of surface antigens, and pluripotency-associated transcription factors, DNA methylation status at pluripotent cell-specific genes, and the capacity to differentiate in vitro and in teratomas. The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage. (Blood. 2009; 113: 5476-5479)