Two simple sequence repeat markers to select for soybean cyst nematode resistance coditioned by the rhg1 locus
Two simple sequence repeat markers to select for soybean cyst nematode resistance coditioned by the rhg1 locus
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DOI：
10.1007/s001220051300
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发表时间：
1999-09-01
影响因子：
5.4
通讯作者：
Young, ND
中科院分区：
文献类型：
--
作者：
Cregan, PB;Mudge, J;Young, ND
大豆胞囊线虫（SCN）（Heterodera大豆）是最具经济意义的大豆害虫。减少或消除这种害虫损害的主要策略是使用抗性品种。在育种计划中鉴定抗性分离子是一个困难且昂贵的过程，由于抗性的寡基因性质和病原体的遗传变异性，该过程变得复杂。幸运的是，一个SCN抗性位点rhg1的抗性被普遍认为是使用任何抗性来源并在受到任何SCN种族挑战时发展抗性基因型的必要条件。因此，如果有一个有效且快速的系统可以鉴定在 rhg1 位点携带抗性等位基因的育种系，那么 SCN 抗性品种的开发将会加速。在这项研究中，我们报告了两个简单序列重复（SSR）或微卫星位点，它们与 rhg1 共分离并定位 0.4 cM。这些基因座中第一个位点 BARC-Satt309 的等位基因变异将大多数（如果不是全部）SCN 易感基因型与在 rhg1 上携带抗性的基因型区分开来，这些基因型源自重要的 SCN 抗性来源“Peking”、PI 437654 和 PI 90763。BARC-Satt309 在区分 SCN 抗性来源 PI 88788 和 PI 方面也有效。 209332 来自许多（但不是全部）易感基因型。 BARC-Satt309 不能用于由典型美国南部品种与重要抗性来源 PI 88788 或 PI 209332 杂交开发的群体的标记辅助选择，因为这些基因型在 BARC-Satt309 基因座上均携带相同的等位基因。第二个 SSR 基因座 BARC-Sat_168 是从使用 BARC-Satt309 引物鉴定的细菌人工染色体 (BAC) 克隆开发而来。 BARC-Sat_168 将 PI 88788 和 PI 209332 与美国南部品种“Lee”、“Bragg”和“Essex”区分开来。 BARC-Satt309 和 BARC-Sat_168 均用于分析来自 SCN 易感性 x SCN 抗性杂交的品系，并被证明能够非常有效地从携带 rhg1 基因座 SCN 易感性等位基因的品系中识别出携带 rhg1 抗性的品系。
The soybean cyst nematode (SCN) (Heterodera glycines Inchinoe) is the most economically significant soybean pest. The principal strategy to reduce or eliminate damage from this pest is the use of resistant cultivars. Identifying resistant segregants in a breeding program is a difficult and expensive process which is complicated by the oligogenic nature of the resistance and genetic variability in the pathogen. Fortunately, resistance at one SCN-resistance locus, rhg1, is generally accepted as a necessity for the development of resistant genotypes using any source of resistance and when challenged by any SCN race. Thus, the development of SCN resistant cultivars would be expedited if an effective and rapid system were available to identify breeding lines carrying a resistance allele at the rhg1 locus. In this study we report two simple sequence repeat (SSR) or microsatellite loci that cosegregate and map 0.4 cM from rhg1. Allelic variation at the first of these loci, BARC-Satt309, distinguished most, if not all, SCN-susceptible genotypes from those carrying resistance at rhg1 derived from the important SCN-resistance sources 'Peking', PI 437654, and PI 90763. BARC-Satt309 was also effective in distinguishing SCN resistance sources PI 88788 and PI 209332 from many, but not all, susceptible genotypes. BARC-Satt309 cannot be used in marker-assisted selection in populations developed from typical southern US cultivars crossed with the important resistance sources PI 88788 or PI 209332 because these genotypes all carry the identical allele at the BARC-Satt309 locus. A second SSR locus, BARC-Sat_168, was developed from a bacterial artificial chromosome (BAC) clone that was identified using the primers to BARC-Satt309. BARC-Sat_168 distinguished PI 88788 and PI 209332 from southern US cultivars such as 'Lee','Bragg' and 'Essex'. Both BARC-Satt309 and BARC-Sat_168 were used to assay Lines from SCN-susceptiblexSCN-resistant crosses and proved to be highly effective in identifying lines carrying rhg1 resistance from those carrying the allele for SCN susceptibility at the rhg1 locus.