Clinicopathologic features and prognostic implications of Gankyrin protein expression in non-small cell lung cancer
Clinicopathologic features and prognostic implications of Gankyrin protein expression in non-small cell lung cancer
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DOI：
10.1016/j.prp.2015.09.010
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发表时间：
2015-01-01
影响因子：
2.8
通讯作者：
Li, Xiao-Fei
中科院分区：
文献类型：
--
作者：
Wang, Wu-ping;Yan, Xiao-long;Li, Xiao-Fei
目的：Gankyrin是一种与肝癌相关的癌蛋白，在包括非小细胞肺癌（NSCLC）在内的多种人类恶性肿瘤中均有表达。方法：采用免疫组化（IHC）法检测166例配对石蜡包埋的NSCLC组织及癌旁正常组织中Gankyrin的表达。采用半定量逆转录聚合酶链反应（RT-PCR）和Western blotting方法检测24例新鲜NSCLC组织及相应正常组织中Gankyrin的表达。还评估了Gankyrin表达与临床病理参数的相关性。Kaplan-Meier生存分析和考克斯比例风险模型用于评估Gankyrin表达对生存的影响。结果：数据显示Gankyrin在78.3%（130/166）的癌组织和28.9%（48/166）的相应癌旁正常组织中表达。肿瘤组织中Gankyrin过表达率为53.6%（89/166），而正常组织中Gankyrin过表达率仅为4.8%（8/166）（P < 0.001）。半定量RT-PCR和Western blotting检测结果显示，NSCLC组织中Gankyrin mRNA和蛋白的表达均高于相应的正常组织。Gankyrin过表达与淋巴结转移（P < 0.001）和p-TNM分期（P < 0.001）显著相关。Gankyrin过表达的NSCLC患者的生存期明显缩短（P < 0.001，Log-rank检验），Gankyrin过表达在鳞癌（P=0.028）和腺癌（P < 0.001）中的预后意义均明显。多因素分析显示Gankyrin过表达可能是NSCLC的一个独立预后因素（HR 1.51; P=0.041）。结论：Gankyrin过表达在NSCLC中具有临床意义，可作为一个预后生物标志物。(C)2015 Elsevier GmbH. All rights reserved.
Purpose: The expression of Gankyrin, a liver cancer-related oncoprotein, has been observed in several human malignancies including non-small cell lung cancer (NSCLC). However, the clinic relevance of Gankyrin expression in NSCLC remains unclear.Methods: Gankyrin expression was assessed using immunohistochemical (IHC) methods in 166 paired paraffin-embedded NSCLC specimens and adjacent normal tissues. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were employed to measure the expression of Gankyrin in 24 paired fresh NSCLC specimens and the corresponding normal tissues. The association of Gankyrin expression with clinicopathological parameters was also evaluated. Kaplan-Meier survival analysis and Cox proportional hazards models were used to estimate the effect of Gankyrin expression on survival.Results: Data showed that Gankyrin was expressed in 78.3% (130/166) and 28.9% (48/166) of cancer lesions and corresponding adjacent normal tissue, respectively. And the Gankyrin overexpression in tumor tissue occurred in 53.6% (89/166) of patients, while overexpression of Gankyrin in normal tissue occurred only in 4.8% (8/166) of patients (P < 0.001). Semi-quantitative RT-PCR and Western blotting showed that NSCLC specimens had increased Gankyrin mRNA and protein expression compared to the corresponding normal tissues. Out of all the clinicopathological factors analyzed, Gankyrin overexpression was significantly correlated with lymphatic metastasis (P < 0.001) and p-TNM stage (P < 0.001). Gankyrin-overexpressed NSCLC patients had a significantly shorter survival time (P < 0.001, Log-rank test), and the prognostic significance of Gankyrin overexpression was apparent in both squamous cell carcinoma patients (P=0.028) and adenocarcinoma patients (P < 0.001). Multivariate analysis indicated that Gankyrin overexpression may be an independent prognostic factor in NSCLC (hazard ratio [HR], 1.51; P=0.041).Conclusion: Our results indicate that Gankyrin overexpression is of clinical significance and can serve as a prognostic biomarker in NSCLC. (C) 2015 Elsevier GmbH. All rights reserved.