Prolonged survival of thymoma-bearing mice after vaccination with a soluble protein antigen entrapped in liposomes: a model study.
Prolonged survival of thymoma-bearing mice after vaccination with a soluble protein antigen entrapped in liposomes: a model study.
复制标题
DOI：
--
复制
发表时间：
1992-11
期刊：
影响因子：
11.2
通讯作者：
F. Zhou;B. Rouse;Leaf Huang;Leaf Huang
中科院分区：
文献类型：
--
作者：
F. Zhou;B. Rouse;Leaf Huang;Leaf Huang
EG 7-OVA细胞是用鸡卵清蛋白（OVA）的互补DNA稳定转染的小鼠胸腺瘤EL 4细胞，因此表达作为独特抗原的OVA表位。卵清蛋白特异性细胞毒性T淋巴细胞可以通过免疫小鼠诱导与卵清蛋白抗原加载到同源脾细胞或包埋在脂质体。由此诱导的细胞毒性T淋巴细胞可以在体外以抗原特异性和主要组织相容性复合物限制的方式特异性地裂解EG 7-OVA细胞。在本研究中，我们已经在这个模型系统中检查了用脂质体OVA免疫是否可以保护小鼠免受EG 7-OVA细胞诱导的肿瘤。用包埋在脂质体中的OVA或在同系脾细胞中免疫加载的OVA接种延长了用EG 7-OVA细胞攻击的小鼠的存活，但不延长用亲本EL 4细胞攻击的小鼠的存活。抗肿瘤作用归因于诱导的OVA特异性细胞毒性T淋巴细胞活性，因为不能检测到其他形式的获得性免疫，例如肿瘤细胞与特异性抗体的相互作用。我们的研究结果表明，免疫与抗原纳入脂质体可能是一个有用的手段，诱导保护性抗肿瘤反应。
EG7-OVA cells are mouse thymoma EL4 cells stably transfected with the complementary DNA of chicken ovalbumin (OVA) and thus express OVA epitopes as a unique antigen. Cytotoxic T lymphocytes specific to OVA can be elicited by immunization of mice with OVA osmotically loaded into syngeneic splenocytes or entrapped in liposomes. Cytotoxic T lymphocytes thus induced can specifically cytolyse the EG7-OVA cells in vitro in an antigen-specific and major histocompatibility complex-restricted manner. In the present study, we have examined in this model system whether immunization with liposomal OVA can protect mice against tumors induced by EG7-OVA cells. Vaccination with OVA either entrapped in liposomes or osmotically loaded in the syngeneic splenocytes prolonged the survival of mice which had been challenged with EG7-OVA cells, but not those mice challenged with the parent EL4 cells. The antitumor effect was attributed to the induced OVA-specific cytotoxic T lymphocyte activity, since other forms of acquired immunity such as interaction of tumor cells with specific antibody could not be detected. Our results demonstrate that immunization with antigen incorporated in liposomes could be a useful means of inducing a protective antitumor response.