MATHEMATICAL-MODEL FOR TIME-RESOLVED AND FREQUENCY-DOMAIN FLUORESCENCE SPECTROSCOPY IN BIOLOGICAL TISSUE
MATHEMATICAL-MODEL FOR TIME-RESOLVED AND FREQUENCY-DOMAIN FLUORESCENCE SPECTROSCOPY IN BIOLOGICAL TISSUE
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DOI：
10.1364/ao.33.001963
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发表时间：
1994-04-01
期刊：
影响因子：
1.9
通讯作者：
POGUE, BW
中科院分区：
文献类型：
--
作者：
PATTERSON, MS;POGUE, BW
一般来说，不可能写出由分布在散射介质（如组织）中的荧光团产生的荧光信号的解析表达式。然而，通过假设组织的散射特性在激发和发射波长上是相同的，我们推导出了荧光和散射信号之间的简单关系。结合扩散理论，用它来写出在组织表面检测到的荧光信号在时间域和频率域的表达式。在组织模拟材料中使用荧光团氯磺化酞菁铝的实验证实了该模型的准确性。讨论了在体内光谱中的应用。
In general it is not possible to write an analytic expression for the fluorescence signal generated by a fluorophore distributed in a scattering medium such as tissue. However, by assuming that the scattering properties of the tissue are the same at the excitation and emission wavelengths, we have derived a simple relation between the fluorescence and the scatter signals. Along with diffusion theory, this was used to write expressions for the fluorescence signal detected at the tissue surface in both the time and the frequency domains. Experiments using the fluorophore aluminum chlorosulfonated phthalocyanine in tissue-simulating materials confirmed the accuracy of the model. Applications to in vivo spectroscopy are discussed.