Spotsizer: High-throughput quantitative analysis of microbial growth.
Spotsizer: High-throughput quantitative analysis of microbial growth.
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DOI：
10.2144/000114459
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发表时间：
2016-10-01
期刊：
影响因子：
2.7
通讯作者：
Bähler J
中科院分区：
文献类型：
--
作者：
Bischof L;Převorovský M;Rallis C;Jeffares DC;Arzhaeva Y;Bähler J
微生物菌落生长可以作为一个有用的读数在分析研究复杂的遗传相互作用或化学化合物的影响。虽然已经开发了用于获取微生物菌落的定量测量的计算工具，但是它们的效用可能受到不灵活的输入图像要求、非平凡的安装程序或复杂操作的影响。在这里，我们提出了Spotsizer软件工具，用于在机器人排列的微生物菌落图像中自动测量菌落大小。Spotsizer具有方便的图形用户界面（GUI），具有单图像和批处理能力，并与多种输入图像格式和不同的菌落网格类型一起工作。我们演示了如何Spotsizer可以用于高通量的裂变酵母生长的定量分析。用户友好的Spotsizer工具以高通量形式提供对微生物生长的快速、准确和强大的定量分析。Spotsizer可在https://data.csiro.au/dap/landingpage?上免费获得pid=csiro：15330在专有的CSIRO许可下。
Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.