The Lotus japonicus nucleoporin GLE1 is involved in symbiotic association with rhizobia
The Lotus japonicus nucleoporin GLE1 is involved in symbiotic association with rhizobia
复制标题
百脉根核孔蛋白 GLE1 与根瘤菌共生
DOI：
10.1111/ppl.12996
复制
发表时间：
2020
影响因子：
6.4
通讯作者：
Suganuma N
中科院分区：
文献类型：
--
作者：
Imai A;Ohtani M;Nara A;Tsukakoshi A;Narita A;Hirakawa H;Sato S;Suganuma N
核孔蛋白是核孔复合物的组成部分，是调节细胞核和细胞质之间大分子运输的通道。核孔蛋白GLE1 （GLFG lethal1）在含有poly(A)尾的信使rna从细胞核输出到细胞质中起作用。本文研究了一种GLE1基因缺陷的模式豆科植物——日本豆科植物，我们将其命名为ljgle1。在与根瘤菌共生的情况下，fljgle1的生长受到阻碍，根瘤的固氮活性约为野生型植物的三分之一。补施复合氮后，fljgle1的生长恢复不明显。在jgle1型上形成的结节比野生型小，呈淡粉色。与野生型相比，jgle1的根瘤感染细胞数量较少，且根瘤细胞未发育。jgle1细胞内根瘤菌形态紊乱，共生体膜与细菌膜紧密附着。这些结果表明，GLE1在豆科植物与根瘤菌的共生关系中起着明显的作用。
Nucleoporins are components of the nuclear pore complexes, channels that regulate the transport of macromolecules between the nucleus and cytoplasm. The nucleoporin GLE1 (GLFG lethal1) functions in the export of messenger RNAs containing poly(A) tails from the nucleus into the cytoplasm. Here we investigated a mutant of the model legumeLotus japonicus that was defective in GLE1, which we designatedLjgle1. The growth ofLjgle1was retarded under symbiotic association with rhizobia, and the nitrogen‐fixation activities of the nodules were around one‐third of those in the wild‐type plant. The growth ofLjgle1was not substantialy recovered by supplemention of combined nitrogen. Nodules formed on theLjgle1were smaller than those on the wild‐type and colored faint pink. The numbers of infected cells of nodules on theLjgle1were smaller than on the wild‐type plant, and the former cells remained undeveloped. Rhizobia in the cells of theLjgle1exhibited disordered forms, and the symbiosome membrane was closely attached to the bacterial membrane. These results indicate that GLE1 plays a distinct role in the symbiotic association between legumes and rhizobia.