Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats
Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats
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DOI：
10.1128/mcb.18.12.6879
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发表时间：
1998-12-01
影响因子：
5.3
通讯作者：
Osborne, HB
中科院分区：
文献类型：
--
作者：
Audic, Y;Omilli, F;Osborne, HB
非洲爪蟾胚胎中母体mRNA的去腺苷化是一个序列特异性过程。一个在受精后靶向母体mRNA进行去腺苷化的顺式元件是胚胎去腺苷化元件（EDEN）。该元件由U/R重复序列组成，被蛋白质EDEN-BP特异性结合。在本研究中，我们表明，含有EDEN的RNA去腺苷化的速率可以增加由三个AUU重复组成的额外的顺式元件的存在下。对于天然EDEN（c-mos）和两种合成EDEN观察到这种效应。因此，由（AUU）基序赋予的EDEN依赖性去腺苷化的增强不是由于与特定EDEN序列的相互作用。（AUU）基序的突变消除了EDEN依赖性去腺苷化的增强。这些数据表明，在一个特定的母体mRNA在非洲爪蟾胚胎deadenylated率可能是由多个顺式元件之间的串扰定义。
The deadenylation of maternal mRNAs in the Xenopus embryo is a sequence-specific process. One cis element that targets maternal mRNAs for deadenylation after fertilization is the embryo deadenylation element (EDEN), This element, composed of U/R repeats, is specifically bound by a protein, EDEN-BP. In the present study we show that the rate at which an RNA containing an EDEN is deadenylated can be increased by the presence of an additional cis element composed of three AUU repeats. This effect was observed for a natural EDEN (c-mos) and two synthetic EDENs, Hence, the enhancement of EDEN-dependent deadenylation conferred by the (AUU), motif is not due to an interaction with a particular EDEN sequence. Mutation of the (AUU), motif abrogated the enhancement of EDEN-dependent deadenylation. These data indicate that the rate at which a specific maternal mRNA is deadenylated in Xenopus embryos is probably defined by a cross talk between multiple cis elements.