The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers
The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers
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DOI：
10.1016/j.biomaterials.2008.09.048
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发表时间：
2009-01-01
期刊：
影响因子：
14
通讯作者：
Granja, Pedro L.
中科院分区：
文献类型：
--
作者：
Barrias, Cristina C.;Martins, M. Cristina L.;Granja, Pedro L.
本研究的目的是比较两个关键的细胞粘附蛋白，纤连蛋白（FN）和玻连蛋白（VN），吸附到分子设计的模型表面的生物学效应。制备了具有OH和CH（3）端基的单组分和混合自组装单分子膜（SAMs），其表面的OH为100%、65%、36%和0%，以产生具有简单化学和润湿性梯度的一系列表面。在非竞争性（单蛋白溶液）和竞争性（多蛋白溶液）条件下吸附FN和VN，并在不同水平上进行比较：吸附量（放射性标记），洗脱，细胞结合结构域的功能介绍（ELISA）和介导细胞粘附的作用（基于抗体的测定）。观察到的趋势与间充质干细胞在粘附和整体细胞形态方面的反应有关。在非竞争性条件下，这两种蛋白质的吸附增加与表面疏水性。竞争性蛋白质的存在显着降低了吸附量，尽管在所有自组装膜中仍然检测到这两种蛋白质。FN的吸附遵循类似于非竞争性条件的趋势，而VN的吸附在100%OH-SAMs上更高。关于洗脱，吸附VN的保留总是高于FN。对于这两种蛋白质，细胞结合结构域的功能呈递在更亲水的100% OH-SAM上更有效。这一事实，再加上这种类型的SAM在竞争性血清蛋白存在下选择性募集和保留VN的能力，似乎与在这些表面上观察到的较好的细胞应答相关，与疏水性0% OH（100% CH（3））-SAM相比。(C)2008爱思唯尔有限公司保留所有权利。
The objective of this Study was to compare the biological effects of two key cell-adhesive proteins, fibronectin (FN) and vitronectin (VN), upon adsorption onto molecularly-designed model surfaces. Single-component and mixed self-assembled monolayers (SAMs) of alkanethiols on gold with OH and CH(3) terminal groups were prepared at 100%, 65%, 36% and 0% of OH at the surface, to generate a range of Surfaces with a simple chemistry and a wettability gradient. FN and VN were adsorbed under noncompetitive (single-protein solutions) and competitive (multi-protein solutions) conditions, and compared at different levels: adsorbed amount (radiolabelling), elution, functional presentation of cell-binding domains (ELISA), and role in mediating cell adhesion (antibody-based assay). The observed trends were related to mesenchymal stem cell response in terms of adhesion and overall cell morphology. Under non-competitive conditions, adsorption of both proteins increased with surface hydrophobicity. The presence of competitive proteins significantly decreased the adsorbed amounts, although both proteins were still detected in all SAMs. Adsorption of FN followed a trend similar to that of non-competitive conditions, while adsorption of VN was higher on 100%OH-SAMs. Concerning elution, retention of adsorbed VN was always higher than that of FN. For both proteins, functional presentation of cell-binding domains was more effective on the more hydrophilic 100%OH-SAMs. This fact, coupled to the ability of this type of SAMs to selectively recruit and retain VN in the presence of competitive serum proteins, seems to correlate with the better cell response observed on these Surfaces, as compared with hydrophobic O%OH(100%CH(3))-SAMs. (C) 2008 Elsevier Ltd. All rights reserved.