Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
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DOI：
10.1007/978-1-61779-207-6_4
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发表时间：
2011-01-01
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影响因子：
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通讯作者：
Wells, Claire M.
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文献类型：
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作者：
Chaubey, Sanjay;Ridley, Anne J.;Wells, Claire M.
细胞的定向迁移（趋化性）不仅发生在伤口愈合和炎症反应期间，而且发生在胚胎发育期间。然而，使细胞能够检测到化学引诱物并随后向来源迁移的细胞内信号传导途径尚未明确定义。Dunn趋化性室与延时显微镜结合是一种强大的工具，使用户能够直接观察细胞对化学引诱物的形态反应，这是真实的时间。在这里，使用邓恩趋化室，我们描述了小鼠骨髓来源的巨噬细胞集落刺激因子-1的反应。这是一个特别有用的协议，因为它可以适用于研究骨髓衍生的巨噬细胞分离自遗传修饰的小鼠，从而研究细胞迁移和趋化性的特定蛋白质的需求。
The directed migration of cells (chemotaxis) occurs not only during wound healing and inflammatory responses but also during embryonic development. However, the intracellular signaling pathways that enable a cell to detect a chemoattractant and subsequently migrate toward the source are not clearly defined. The Dunn chemotaxis chamber in conjunction with time-lapse microscopy is a powerful tool that enables the user to observe directly the morphological response of cells to a chemoattractant in real time. Here, using the Dunn chemotaxis chamber, we describe the response of murine bone marrow-derived macrophages to colony stimulating factor-1. This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement of a specific protein in cell migration and chemotaxis.