DNA binding to electropolymerized polypyrrole: The dependence on film characteristics
DNA binding to electropolymerized polypyrrole: The dependence on film characteristics
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DOI：
10.1081/ma-100108381
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发表时间：
2001-01-01
期刊：
影响因子：
--
通讯作者：
Tripathy, SK
中科院分区：
文献类型：
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作者：
Minehan, DS;Marx, KA;Tripathy, SK
证据表明，DNA的电聚合聚吡咯（PPy）的结合是直接相关的正电荷载流子在PPy的存在下。短的放射性标记的OX174 DNA片段（平均大小为300 bp）的吸附速率上掺杂对甲苯磺酸的电氧化聚吡咯薄膜被发现作为一个函数的变化。薄膜储存条件、薄膜老化、电化学还原，但不影响合成电流密度。DNA结合更容易在干燥条件下储存的电影。DNA结合仅随PPy电聚合过程中使用的电流密度略有变化。随着PPy膜龄的增加，DNA结合水平显著降低。这一观察定性相关，但不定量，与已知的损失的聚吡咯导电性和电荷缺陷与老化。DNA吸附水平下降的速度远远超过电影年龄比电导率。氧化聚吡咯膜结合DNA在更高的速率比膜已减少后，电氧化合成，从而消除了大部分的表面正电荷缺陷。这些结果支持的想法，DNA结合所带来的正电荷缺陷载流子的存在下，在聚吡咯膜。
Evidence is presented that suggests the binding of DNA to electropolymerized polypyrrole (PPy) is directly related to the presence of positive charge carriers in PPy. The adsorption rate of short radiolabelled OX174 DNA fragments (average size 300 bp) onto thin films of electroxidized PPy doped with p-toluene sulfonate was found to vary as a function of. film storage conditions, film age, electrochemical reduction, but not synthesis current density. DNA bound more readily to films stored under dry conditions. DNA binding varied only slightly with the current density used during PPy electropolymerization. The DNA binding levels were significantly reduced with increasing PPy film age. This observation correlated qualitatively, but not quantitatively, with the known loss of PPy conductivity and charge defects with aging. DNA adsorption levels fell off far more rapidly with film age than does conductivity. Oxidized PPy films bound DNA at a higher rate than films which had been reduced subsequent to electroxidative synthesis, thereby removing a large fraction of the surface positive charge defects. These results support the idea that DNA binding is brought about by the presence of positive charge defect carriers in the PPy film.