Method for the measurement of antioxidant activity in human fluids
Method for the measurement of antioxidant activity in human fluids
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DOI：
10.1136/jcp.54.5.356
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发表时间：
2001-05-01
影响因子：
3.4
通讯作者：
Cosic, V
中科院分区：
文献类型：
--
作者：
Koracevic, D;Koracevic, G;Cosic, V
目的 - 开发一种新的、简单且廉价的方法来估计人体体液中的抗氧化活性。方法 - 该测定测量了生物体液在芬顿反应产生的自由基的影响下抑制苯甲酸钠产生硫代巴比妥酸反应物质 (TBARS) 的能力。使用1毫摩尔/升尿酸溶液作为标准。 结果-在各种生物液体中发现以下平均(SD)抗氧化活性(尿酸)：血清，2.04(0.20)毫摩尔/升；尿液，176.5（25.6）μmol/升；脑脊液，95.0（26.9）μmol/升；眼房水，61.25（9.9）μmol/升；唾液，838.5（48.2）μmol/升；泪液，247.0（17.0）μmol/升；腹水，270.0（63.3）μmol/升；肾囊肿液，387.1（28.1）μmol/升。分析需要少量生物材料样品：10μl血清和50-100μl其他体液。在 48 名健康个体的血清中，使用 Randox 方法（作为参考方法）获得的值与此处提出的新方法之间存在显着的正相关性（相关系数，0.8728；方法之间的平均差异，
Aim-To develop a new, simple, and cheap method for estimating antioxidant activity in human fluids.Methods-The assay measured the capacity of the biological fluids to inhibit the production of thiobarbituric acid reactive substances (TBARS) from sodium benzoate under the influence of the free oxygen radicals derived from Fenton's reaction. A solution of 1 mmol/litre uric acid was used as standard.Results-The following mean (SD) antioxidative activities were found las uric acid) in the various biological fluids: serum, 2.04 (0.20) mmol/litre; urine, 176.5 (25.6) mu mol/litre; cerebrospinal fluid, 95.0 (26.9) mu mol/litre; aqueous humour oculi, 61.25 (9.9) mu mol/litre; saliva, 838.5 (48.2) mu mol/litre; tears, 247.0 (17.0) mu mol/ Litre; ascites fluid, 270.0 (63.3) mu mol/litre; kidney cyst fluid, 387.1 (28.1) mu mol/litre. Small samples of the biological material were needed for the analyses: 10 mul of serum and 50-100 mul Of Other body fluids. In the sera of 48 healthy individuals there was a significant positive correlation between values obtained with the Randox method las a reference method) and the new method proposed here (correlation coefficient, 0.8728; mean difference between methods,