Effects of Cα-oxidation in the fungal metabolism of lignin
Effects of Cα-oxidation in the fungal metabolism of lignin
复制标题
Cα-氧化对木质素真菌代谢的影响
DOI：
10.1080/02773818408081148
复制
发表时间：
1984
影响因子：
2
通讯作者：
T. Kirk
中科院分区：
文献类型：
--
作者：
P. Fenn;T. Kirk
摘要Cα-氧化（苯甲醇氧化）是白腐菌降解木质素的一个重要反应。研究表明，这种氧化作用显著地抑制了黄孢原毛平革菌培养物对非酚类β-O-4模型化合物1-（3-甲氧基-4-乙氧苯基）-2-（邻甲氧基苯氧基）丙烷-1，3-二醇的代谢。然而，令人惊讶的是，云杉木质素的选择性化学Cα -氧化增强了其解聚的文化。因此，木质素中Cα-氧化的另一种作用超过了亚结构内在降解性的降低。一种可能性是氧化通过降低其空间复杂性来增加木质素对酶的可及性。本研究还表明，β-O-4模型，如木材中的木质素，部分通过Cα-氧化被P. chrysosporium降解。由此形成的α-羰基不会发生还原。添加L-谷氨酸木质素降解培养物完全抑制他们的能力，降解的M…
Abstract Cα-Oxidation (benzyl alcohol oxidation) is a prominent reaction in the degradation of lignin by white-rot fungi. This study showed that such oxidation markedly retards metabolism of a nonphenolic β-O-4 model compound, 1-(3-methoxy-4-ethoxyphenyl)-2-(o-methoxyphenoxy)propane-1,3-diol, by cultures of Phanerochaete chrysosporium Burds. Surprisingly, however, selective chemical Cα -oxidation of spruce lignins enhanced their depolymerization by the cultures. Thus the decrease in intrinsic degradability of substructures is more than compensated by another effect of Cα-oxidation in lignin. One possibility is that the oxidation increases the accessibility of the lignin to enzymes by decreasing its steric complexity. This study also revealed that the β-O-4 model, like lignin in wood, is degraded in part via Cα-oxidation by P. chrysosporium. Reduction of the α-carbonyl groups thus formed does not occur. Addition of L-glutamate to ligninolytic cultures completely suppresses their competence to degrade the m...