Clusters of cerebellar Purkinje cells control their afferent climbing fiber discharge
Clusters of cerebellar Purkinje cells control their afferent climbing fiber discharge
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DOI：
10.1073/pnas.1302310110
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发表时间：
2013-10-01
影响因子：
11.1
通讯作者：
Isope, Philippe
中科院分区：
文献类型：
--
作者：
Chaumont, Joseph;Guyon, Nicolas;Isope, Philippe
攀爬纤维是从下橄榄核到小脑皮层的投射，携带感觉运动误差和时钟信号，通过控制小脑浦肯野细胞突触可塑性和放电来触发运动学习。浦肯野细胞以深部小脑核为目标，小脑核是小脑的输出，包括向下橄榄的抑制性 GABA 能投射。该通路确定了橄榄-皮质-核网络中潜在的闭环。因此，浦肯野细胞组可能会阶段性地控制它们自己的攀爬纤维传入。在这里，利用体外和体内记录，我们描述了一种转基因小鼠模型，该模型允许对浦肯野细胞放电进行特定的光遗传学控制。小脑核中的四极管记录表明，浦肯野细胞的局灶刺激强烈抑制空间受限的小脑核神经元组。引人注目的是，这种刺激会在受刺激的浦肯野细胞中触发延迟的攀爬纤维输入信号。因此，我们的结果表明，浦肯野细胞阶段性地控制其自身橄榄传入神经的放电，因此可能参与小脑运动学习的调节。
Climbing fibers, the projections from the inferior olive to the cerebellar cortex, carry sensorimotor error and clock signals that trigger motor learning by controlling cerebellar Purkinje cell synaptic plasticity and discharge. Purkinje cells target the deep cerebellar nuclei, which are the output of the cerebellum and include an inhibitory GABAergic projection to the inferior olive. This pathway identifies a potential closed loop in the olivo-cortico-nuclear network. Therefore, sets of Purkinje cells may phasically control their own climbing fiber afferents. Here, using in vitro and in vivo recordings, we describe a genetically modified mouse model that allows the specific optogenetic control of Purkinje cell discharge. Tetrode recordings in the cerebellar nuclei demonstrate that focal stimulations of Purkinje cells strongly inhibit spatially restricted sets of cerebellar nuclear neurons. Strikingly, such stimulations trigger delayed climbing-fiber input signals in the stimulated Purkinje cells. Therefore, our results demonstrate that Purkinje cells phasically control the discharge of their own olivary afferents and thus might participate in the regulation of cerebellar motor learning.