Size distributions of maternal and fetal DNA in maternal plasma
Size distributions of maternal and fetal DNA in maternal plasma
复制标题
DOI：
10.1373/clinchem.2003.024893
复制
发表时间：
2004-01-01
影响因子：
9.3
通讯作者：
Lo, YMD
中科院分区：
文献类型：
--
作者：
Chan, KCA;Zhang, J;Lo, YMD
背景：孕妇血浆中胎儿DNA的发现为无创性产前诊断开辟了一条途径。尽管在临床应用中的快速扩张，血浆DNA在孕妇的分子特征仍不清楚。方法：我们调查了血浆DNA的大小分布在34名非孕妇和31名孕妇，使用一个面板的定量PCR检测与不同的扩增子大小针对瘦素基因。结果：孕妇和非孕妇血浆中大于201 bp的DNA的中位数分别为57%和14%（P < 0.05），孕妇血浆中大于193 bp和大于313 bp的DNA的中位数分别为20%和0%（P < 0.05）。孕妇血浆中的DNA片段明显长于非孕妇血浆中的DNA片段，并且母体来源的DNA分子长于胎儿来源的DNA分子。(C)2004年美国临床化学协会。
Background: The discovery of fetal DNA in maternal plasma has opened up an approach for noninvasive prenatal diagnosis. Despite the rapid expansion in clinical applications, the molecular characteristics of plasma DNA in pregnant women remain unclear.Methods: We investigated the size distribution of plasma DNA in 34 nonpregnant women and 31 pregnant women, using a panel of quantitative PCR assays with different amplicon sizes targeting the leptin gene. We also determined the size distribution of fetal DNA in maternal plasma by targeting the SRY gene.Results: The median percentages of plasma DNA with size >201 bp were 57% and 14% for pregnant and nonpregnant women, respectively (P 193 bp and >313 bp were 20% and 0%, respectively, in maternal plasma.Conclusion: Plasma DNA molecules are mainly short DNA fragments. The DNA fragments in the plasma of pregnant women are significantly longer than those in the plasma of nonpregnant women, and the maternal-derived DNA molecules are longer than the fetal-derived ones. (C) 2004 American Association for Clinical Chemistry.