Transcriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO3-induced organic acid secretion in grapevine roots
Transcriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO3-induced organic acid secretion in grapevine roots
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转录组学和磷酸化蛋白质组学分析以及代谢物分析揭示了 NaHCO3 诱导葡萄根中有机酸分泌的机制
DOI：
10.1186/s12870-019-1990-9
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发表时间：
2019-09
影响因子：
5.3
通讯作者：
Yuxin Yao
中科院分区：
文献类型：
--
作者：
Guangqing Xiang;Wanyun Ma;Shiwei Gao;Zhongxin Jin;Qianyu Yue;Yuxin Yao
背景有机酸分泌是植物对碱度的一种广泛的生理反应。结果草酸是山葡萄(Vitis amurensis，V.berlandieriandV.)的杂交种葡萄合成和分泌的主要有机酸。在NaHCO3胁迫下，醋酸酯合成和苹果酸分泌也被促进。NaHCO3胁迫提高了葡萄根系H+流出速率，这与质膜H+-ATPase活性有关。转录图谱显示，碳水化合物代谢是NaHCO3胁迫下最显著的生物过程；共有7个与有机酸代谢相关的基因发生了显著变化，其中包括两个磷酸烯醇式丙酮酸羧化酶和两个磷酸烯醇式丙酮酸羧化酶。此外，NaHCO3胁迫显著上调了5个ATP结合盒转运体的表达水平，特别是ATP结合盒B19和2个铝激活的苹果酸转运体2 的表达水平。磷蛋白组学分析表明，改变的磷蛋白主要与其分子功能的结合、催化活性和转运蛋白活性有关。磷酸烯醇式丙酮酸羧化酶3、两种质膜H+-ATPase 4和三磷酸腺苷结合盒B19的磷酸化水平及多效性耐药12显著增加。此外，抑制乙烯合成和感知完全阻断了NaHCO3诱导的有机酸分泌，而抑制吲哚乙酸合成则减少了NaHCO3诱导的有机酸分泌。结论本研究结果表明，草酸是碱胁迫下产生的主要有机酸，揭示了乙烯在调节有机酸分泌中的必要性。此外，我们还进一步鉴定了几个与有机酸代谢和分泌有关的候选基因和磷蛋白。
BackgroundOrganic acid secretion is a widespread physiological response of plants to alkalinity. However, the characteristics and underlying mechanism of the alkali-induced secretion of organic acids are poorly understood.ResultsOxalate was the main organic acid synthesized and secreted in grapevine (a hybrid ofVitis amurensis,V. berlandieriandV. riparia) roots, while acetate synthesis and malate secretion were also promoted under NaHCO3stress. NaHCO3stress enhanced the H+efflux rate of grapevine roots, which is related to the plasma membrane H+-ATPase activity. Transcriptomic profiling revealed that carbohydrate metabolism was the most significantly altered biological process under NaHCO3stress; a total of seven genes related to organic acid metabolism were significantly altered, including two phosphoenolpyruvate carboxylases and phosphoenolpyruvate carboxylase kinases. Additionally, the expression levels of fiveATP-binding cassette transporters, particularlyATP-binding cassette B19, and twoAl-activated malate transporter 2 swere substantially upregulated by NaHCO3stress. Phosphoproteomic profiling demonstrated that the altered phosphoproteins were primarily related to binding, catalytic activity and transporter activity in the context of their molecular functions. The phosphorylation levels of phosphoenolpyruvate carboxylase 3, two plasma membrane H+-ATPases 4 and ATP-binding cassette B19 and pleiotropic drug resistance 12 were significantly increased. Additionally, the inhibition of ethylene synthesis and perception completely blocked NaHCO3-induced organic acid secretion, while the inhibition of indoleacetic acid synthesis reduced NaHCO3-induced organic acid secretion.ConclusionsOur results demonstrated that oxalate was the main organic acid produced under alkali stress and revealed the necessity of ethylene in mediating organic acid secretion. Additionally, we further identified several candidate genes and phosphoproteins responsible for organic acid metabolism and secretion.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
10.1073/pnas.1613499114
发表时间：
2017-06-13
影响因子：
11.1
作者：
Barbez, Elke;Duenser, Kai;Busch, Wolfgang
通讯作者：
Busch, Wolfgang
生长素通过上调 GmMATE 和激活大豆根质膜 H -ATP 酶增强铝诱导的柠檬酸渗出
影响因子：
4.2
作者：
Ping Wang;Wenqian Yu;Jiarong Zhang;Zed Rengel;Jin Xu;Qinqin Han;Limei Chen;Kunzhi Li;Yongxiong Yu;Qi Chen
通讯作者：
Qi Chen
影响因子：
11.6
作者：
Ladwig, Friederike;Dahlke, Renate I.;Sauter, Margret
通讯作者：
Sauter, Margret
影响因子：
7.3
作者：
Ligaba, Ayalew;Maron, Lyza;Pineros, Miguel
通讯作者：
Pineros, Miguel