Metabarcoding of Environmental DNA Samples to Explore the Use of Uranium Mine Containment Ponds as a Water Source for Wildlife
Metabarcoding of Environmental DNA Samples to Explore the Use of Uranium Mine Containment Ponds as a Water Source for Wildlife
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对环境 DNA 样本进行元条形码探索将铀矿密封池用作野生动物的水源
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
J. Hinck
中科院分区：
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作者：
K. Klymus;C. Richter;Nathan L. Thompson;J. Hinck
要了解人类对景观的影响如何影响野生动物，需要了解群落组成。物种调查是评估群落结构的第一步，最近的分子应用，如元生物编码和环境DNA分析，已被提出作为补充和补充的野生动物调查方法。在这里，我们测试Edna MetabarCoding作为一种调查工具，以检查野生动物将铀矿围隔池塘用作水源的可能性。我们用12S和16S rRNA基因扩增两个标记对矿坑附近的地表水和一个矿坑围隔池塘的样品进行了检测，以调查脊椎动物物种。我们从预计在该地区的分类群以及不太常见或很难观察到的分类群，如老虎、火蜥蜴和灰狐中恢复了大量的序列读数。这两个物种的检测值得注意，因为它们在之前的物种评估中没有被观察到，而且在地雷围隔池塘的样本中发现了老虎蜥蜴的DNA。我们还发现，在这些高度浑浊的地表水中，离心浓缩样品是一种比过滤更有效和更可行的方法。最终，使用EDNA元生物编码可以更好地了解该地区的整体生物多样性和群落组成，并有助于当前的生态毒理风险评估工作。
Understanding how anthropogenic impacts on the landscape affect wildlife requires a knowledge of community assemblages. Species surveys are the first step in assessing community structure, and recent molecular applications such as metabarcoding and environmental DNA analyses have been proposed as an additional and complementary wildlife survey method. Here, we test eDNA metabarcoding as a survey tool to examine the potential use of uranium mine containment ponds as water sources by wildlife. We tested samples from surface water near mines and from one mine containment pond using two markers, 12S and 16S rRNA gene amplicons, to survey for vertebrate species. We recovered large numbers of sequence reads from taxa expected to be in the area and from less common or hard to observe taxa such as the tiger salamander and gray fox. Detection of these two species is of note because they were not observed in a previous species assessment, and tiger salamander DNA was found in the mine containment pond sample. We also found that sample concentration by centrifugation was a more efficient and more feasible method than filtration in these highly turbid surface waters. Ultimately, the use of eDNA metabarcoding could allow for a better understanding of the area’s overall biodiversity and community composition as well as aid current ecotoxicological risk assessment work.