Dietary Selenium Affects Selenoprotein W Gene Expression in the Liver of Chicken
Dietary Selenium Affects Selenoprotein W Gene Expression in the Liver of Chicken
复制标题
日粮硒对鸡肝脏硒蛋白W基因表达的影响
DOI：
10.1007/s12011-011-8995-z
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发表时间：
2011-12-01
影响因子：
3.9
通讯作者：
Xu, Shiwen
中科院分区：
文献类型：
--
作者：
Sun, Bo;Wang, Rihua;Xu, Shiwen
由于“硒蛋白W（SelW）”形式的硒对于维持正常肝功能至关重要，因此肝脏SelW的表达取决于饮食中硒的水平。尽管哺乳动物的情况众所周知，但关于膳食硒对鸟类肝脏中 SelW 表达的影响知之甚少。为了研究日粮硒水平对鸟肝脏中 SelW mRNA 表达的影响，1 日龄的雄性鸡饲喂商业日粮或含有 1.0、2.0、3.0 和 5.0 mg/kg 亚硒酸钠 (Na(2)SeO(3)) 的补硒日粮，持续 90 天。收集肝脏并检查 Se 含量以及 SelW、硒磷酸合成酶 1 和硒代半胱氨酸合成酶 (SecS) 的 mRNA 水平。数据表明，在一定范围内，添加硒的饮食可以增加SelW的表达和SecS的mRNA水平，并且SelW的转录对饮食中的硒非常敏感。
As selenium in the form of "Selenoprotein W (SelW)" is essential for the maintenance of normal liver function, the expression of SelW liver depends on the level of selenium supplied with the diet. Whereas this is well known to be the case in mammals, relatively little is known about the effect of dietary Se on the expression SelW in the livers of avian species. To investigate the effects of dietary Se levels on the SelW mRNA expression in the liver of bird, 1-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0, and 5.0 mg/kg sodium selenite (Na(2)SeO(3)) for 90 days. The livers were collected and examined for Se content and mRNA levels of SelW, Selenophosphate synthetase-1, and selenocysteine-synthase (SecS). The data indicate that, within a certain range, a Se-supplemented diet can increase the expression of SelW and the mRNA levels of SecS, and also, that the transcription of SelW is very sensitive to dietary Se.