HIGH-RESOLUTION SCANNING-ELECTRON MICROSCOPY OF STEREOCILIA USING THE OSMIUM-THIOCARBOHYDRAZIDE COATING TECHNIQUE
HIGH-RESOLUTION SCANNING-ELECTRON MICROSCOPY OF STEREOCILIA USING THE OSMIUM-THIOCARBOHYDRAZIDE COATING TECHNIQUE
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DOI：
10.1016/0378-5955(86)90222-4
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发表时间：
1986-01-01
期刊：
影响因子：
2.8
通讯作者：
HACKNEY, CM
中科院分区：
文献类型：
--
作者：
FURNESS, DN;HACKNEY, CM
使用高分辨率扫描电子显微镜对涂有锇硫代碳酰肼的豚鼠耳蜗进行了对粪纤毛虫详细形态的进一步观察。在立体纤毛之间观察到三种类型的交联。侧对侧和行对行连接由丝状网络组成，而向上的连接是细单链，通常具有末端加宽。静纤毛具有表面。尽管据报道对长期锇固定敏感，但在内毛细胞和外毛细胞上都观察到了这些特征。我们建议锇敏感性可能会因制备过程中使用的缓冲条件而改变。与使用其他扫描电子显微镜制备技术进行的观察相比，对锇涂层材料的观察结果与使用透射电子显微镜进行的观察结果更接近，因为不存在金涂层伪影，并且样品塌陷的程度较小。这使我们能够观察到以前在 SEM 中未报告过的链接及其附件的详细信息。
Further observations on the detailed morphology of sterocilia have been made using high-resolution scanning-electron microscopy of osmium-thiocarbohydrazide-coated guinea pig cochleae. Three types of cross-link have been observed between sterocilia. Side-to-side and row-to-row linkages are composed of a filamentous network whilst upward-pointing links are a fine single strand, often with a terminal widening. The stereocilia have surfaces. These features are observed on both inner and outer hair cells despite reported sensitivity to long periods of osmium fixation. We suggest that osmium sensitivity may be altered by the buffering conditions used during preparation. The observations on osmium-coated material correspond more closely with those made using transmission-electron microscopy than those made using other scanning-electron microscopical preparation techniques, since gold-coating artefacts are absent and the degree of specimen collapse is less. This has enabled us to observe fine details of the links and their attachments which have not been reported previously in SEM.