Topographic Reorganization of Cerebrovascular Mural Cells under Seizure Conditions
Topographic Reorganization of Cerebrovascular Mural Cells under Seizure Conditions
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DOI：
10.1016/j.celrep.2018.03.110
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发表时间：
2018-04-24
期刊：
影响因子：
8.8
通讯作者：
Jeanneteau, Freddy
中科院分区：
文献类型：
--
作者：
Arango-Lievano, Margarita;Boussadia, Badreddine;Jeanneteau, Freddy
在癫痫脑中，神经血管单位的重组已被提出，尽管动力学和功能意义仍不清楚。在这里，我们追踪了癫痫持续状态后血管周围壁细胞的体内动力学，作为脑电图（EEG）活动的函数。我们分割皮质血管床，提供一个特定的尺寸和类型的壁细胞可塑性拓扑分析。我们发现，在癫痫发作诱导后，壁细胞从静脉、小动脉和毛细血管中增加和移除。壁细胞的损失与癫痫发作的严重程度和血管病理学（例如，刚性、灌注和渗透性）。血小板衍生生长因子亚基BB（PDGF-BB）治疗减少了壁细胞丢失、血管病理和癫痫样EEG活动。我们认为，血管周围壁细胞在癫痫发作中起着关键作用，是减少病理生理的潜在靶点。
Reorganization of the neurovascular unit has been suggested in the epileptic brain, although the dynamics and functional significance remain unclear. Here, we tracked the in vivo dynamics of perivascular mural cells as a function of electroencephalogram (EEG) activity following status epilepticus. We segmented the cortical vascular bed to provide a size- and type-specific analysis of mural cell plasticity topologically. We find that mural cells are added and removed from veins, arterioles, and capillaries after seizure induction. Loss of mural cells is proportional to seizure severity and vascular pathology (e.g., rigidity, perfusion, and permeability). Treatment with platelet-derived growth factor subunits BB (PDGF-BB) reduced mural cell loss, vascular pathology, and epileptiform EEG activity. We propose that perivascular mural cells play a pivotal role in seizures and are potential targets for reducing pathophysiology.