Expression of peroxisome proliferator-activated receptors (PPARs) and retinoic acid receptors (RXRs) in rat cortical neurons
Expression of peroxisome proliferator-activated receptors (PPARs) and retinoic acid receptors (RXRs) in rat cortical neurons
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DOI：
10.1016/j.neuroscience.2004.09.043
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Di Loreto, S
中科院分区：
文献类型：
--
作者：
Cimini, A;Benedetti, E;Di Loreto, S
神经元分化是一个复杂的过程，涉及到几个因素的顺序表达。脂质分子在大脑发育中的重要作用是众所周知的。许多脂肪酸细胞信号传导活动由过氧化物酶体增殖物激活受体（PPARs）介导。PPARs是属于类固醇、甲状腺和类维生素A核受体超家族的配体激活的转录因子。它们被脂肪酸及其衍生物激活。PPARs的不同同种型（α、β/δ和γ）具有不同的生理功能，这取决于它们不同的配体活化谱和组织分布。PPARs参与神经细胞分化和死亡以及炎症和神经变性。虽然PPARs已被描述在神经元中的原位研究，这些受体的存在和可能的调制过程中的神经元分化尚未探索。在这项研究中，我们分析了PPARs和他们的异二聚体的合作伙伴，RXRs，在胚胎大鼠皮层神经元在体外成熟过程中的表达。我们的研究结果表明存在PPARs α，β/δ和γ以及RXRs β和γ。PPARalpha、beta/delta和gamma在培养时间期间被差异调节，表明它们可能参与神经元成熟。特别是，我们指出PPARbeta/delta同种型是神经元分化的关键因素。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Neuronal differentiation is a complex process involving the sequential expression of several factors. The important role of lipid molecules in brain development is well known. Many fatty acid cell signaling activities are mediated by peroxisome proliferator-activated receptors (PPARs). PPARs are ligand-activated transcription factors belonging to the steroid, thyroid and retinoid nuclear receptor superfamily. They are activated by fatty acids and their derivatives. Different isotypes of PPARs (alpha, beta/delta and gamma) have distinct physiological functions depending on their different ligand activation profiles and tissue distribution. PPARs have been involved in neural cell differentiation and death as well as in inflammation and neurodegeneration. Although PPARs have been described in neurons by in situ studies, the presence and possible modulation of these receptors during neuronal differentiation has not been explored yet. In this study we analyzed the expression of PPARs and of their heterodimeric partners, RXRs, in embryonic rat cortical neurons during their in vitro maturation. Our results demonstrate the presence of PPARs alpha, beta/delta and gamma and of RXRs beta and gamma. PPARalpha, beta/delta and gamma are differentially modulated during culture time suggesting that they may be involved in neuronal maturation. In particular, we point toward the PPARbeta/delta isotype as a key factor in neuronal differentiation. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.